Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LPIN3 / Lipin-3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110155-100
Catalog Number: LS-G110155
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Lipin 3(LPIN3) (NM_022896), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ620E11.2 | DJ450M14.2 | Lipin-3 | Lipin 3 | Lipin 3-like | Lipin-3-like | LPIN3 | SMP2 | DJ450M14.3 | LIPN3L
- Origin Species
- Human
- SwissProt
- Q9BQK8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 93.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MNYVGQLAET VFGTVKELYR GLNPATLSGG IDVLVVKQVD GSFRCSPFHV RFGKLGVLRS61REKVVDIELN GEPVDLHMKL GDSGEAFFVQ ELESDDEHVP PGLCTSPIPW GGLSGFPSDS121QLGTASEPEG LVMAGTASTG RRKRRRRRKP KQKEDAVATD SSPEELEAGA ESELSLPEKL181RPEPPGVQLE EKSSLQPKDI YPYSDGEWPP QASLSAGELT SPKSDSELEV RTPEPSPLRA241ESHMQWAWGR LPKVARAERP ESSVVLEGRA GATSPPRGGP STPSTSVAGG VDPLGLPIQQ301TEAGADLQPD TEDPTLVGPP LHTPETEESK TQSSGDMGLP PASKSWSWAT LEVPVPTGQP361ERVSRGKGSP KRSQHLGPSD IYLDDLPSLD SENAALYFPQ SDSGLGARRW SEPSSQKSLR421DPNPEHEPEP TLDTVDTIAL SLCGGLADSR DISLEKFNQH SVSYQDLTKN PGLLDDPNLV481VKINGKHYNW AVAAPMILSL QAFQKNLPKS TMDKLEREKM PRKGGRWWFS WRRRDFLAEE541RSAQKEKTAA KEQQGEKTEV LSSDDDAPDS PVILEIPSLP PSTPPSTPTY KKSLRLSSDQ601IRRLNLQEGA NDVVFSVTTQ YQGTCRCKAT IYLWKWDDKV VISDIDGTIT KSDALGHILP661QLGKDWTHQG ITSLYHKIQL NGYKFLYCSA RAIGMADLTK GYLQWVSEGG CSLPKGPILL721SPSSLFSALH REVIEKKPEV FKVACLSDIQ QLFLPHGQPF YAAFGNRPND VFAYRQVGLP781ESRIFTVNPR GELIQELIKN HKSTYERLGE VVELLFPPVA RGPSTDLANP EYSNFCYWRE841PLPAVDLDTL DTRTRPLEQKLISEEDLAANDILDYKDDDDKV
851 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.