Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LOC391003 / PRAMEF18 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119170-100
Catalog Number: LS-G119170
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRAMEF18 (NM_001099850), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PRAME family member 18 | OTTHUMG00000002932 | PRAMEF18
- Origin Species
- Human
- NCBI GenBank
- NM_001099850
- SwissProt
- Q5VWM3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSFQAPRRLL ELAGQSLLRD QALAISVLDE LPRELFPRLF VEAFTSRRCE VLKVMVQAWP61FPCLPLGSLM KTPDLEILHY VVDGIDCLLA QKVRPRRWKL QVLEMRDVDE NFWTIWSGAR121PLSCSPEAMS KRQTVEDCPR TGEKQPLKVF MDVCLKEKSV DEDLSFFSGW VQHRRRSVHL181CCTKVVNYSM NILNFRNILE TVYPDSIQVL EIWNMCWPCM VAEVSRYLSQ MKNLRKLFIS241DGCGYLPSFE SQGQLVAEFS SVFLRLEYLQ MLYMRRIRFF EGYLDQLIRC LKSPLETLAL301TYGSLDEEDL KCLPWYPSLS QLKQLNLSHG TLRFIRLEPL RALLEKVAAT LQTLFLVDCG361IGDSKLRVIL PALSRCSNLT TFCFHGNDTS MDGLKDLLRH TGRLSNLSLE TYPAPRESLD421NRGRVISELL TPLQAELMRI LREVREPNRI FFGPVSCPCC GMSPTEQLEF NFCLRGRPATRTRPLEQKLISEEDLAANDILDYKDDDDKV
479 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.