LSBio
Human LOC391003 / PRAMEF18 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human LOC391003 / PRAMEF18 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G95487-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PRAMEF18 (NM_001099850) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PRAME family member 18 | OTTHUMG00000002932 | PRAMEF18
Origin Species
Human
NCBI GenBank
NM_001099850
SwissProt
Q5VWM3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSFQAPRRLL ELAGQSLLRD QALAISVLDE LPRELFPRLF VEAFTSRRCE VLKVMVQAWP
61FPCLPLGSLM KTPDLEILHY VVDGIDCLLA QKVRPRRWKL QVLEMRDVDE NFWTIWSGAR
121PLSCSPEAMS KRQTVEDCPR TGEKQPLKVF MDVCLKEKSV DEDLSFFSGW VQHRRRSVHL
181CCTKVVNYSM NILNFRNILE TVYPDSIQVL EIWNMCWPCM VAEVSRYLSQ MKNLRKLFIS
241DGCGYLPSFE SQGQLVAEFS SVFLRLEYLQ MLYMRRIRFF EGYLDQLIRC LKSPLETLAL
301TYGSLDEEDL KCLPWYPSLS QLKQLNLSHG TLRFIRLEPL RALLEKVAAT LQTLFLVDCG
361IGDSKLRVIL PALSRCSNLT TFCFHGNDTS MDGLKDLLRH TGRLSNLSLE TYPAPRESLD
421NRGRVISELL TPLQAELMRI LREVREPNRI FFGPVSCPCC GMSPTEQLEF NFCLRGRPA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

479 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.