Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LAMA3 / Laminin Alpha 3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G123324-100
Catalog Number: LS-G123324
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LAMA3 (NM_000227), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BM600 | Epiligrin 170 kDa subunit | E170 | Kalinin-165kDa | LAMA3 | Lama3a | Laminin Alpha 3 | Laminin alpha-3 chain | Laminin-5 alpha 3 chain | Nicein-150kDa | Laminin | alpha 3 | LOCS | Nicein 150kD subunit | BM600 150kD subunit | BM600-150kDa | Epiligrin | Epiligrin alpha 3 subunit | Epiligrin subunit alpha | Kalinin 165kD subunit | Kalinin subunit alpha | Laminin subunit alpha-3 | Laminin-5 subunit alpha | Laminin-6 subunit alpha | Laminin-7 subunit alpha | LAMNA | Nicein subunit alpha
- Origin Species
- Human
- NCBI GenBank
- NM_000227
- SwissProt
- Q16787
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 190.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPPAVRRSAC SMGWLWIFGA ALGQCLGYSS QQQRVPFLQP PGQSQLQASY VEFRPSQGCS61PGYYRDHKGL YTGRCVPCNC NGHSNQCQDG SGICVNCQHN TAGEHCERCQ EGYYGNAVHG121SCRACPCPHT NSFATGCVVN GGDVRCSCKA GYTGTQCERC APGYFGNPQK FGGSCQPCSC181NSNGQLGSCH PLTGDCINQE PKDSSPAEEC DDCDSCVMTL LNDLATMGEQ LRLVKSQLQG241LSASAGLLEQ MRHMETQAKD LRNQLLNYRS AISNHGSKIE GLERELTDLN QEFETLQEKA301QVNSRKAQTL NNNVNRATQS AKELDVKIKN VIRNVHILLK QISGTDGEGN NVPSGDFSRE361WAEAQRMMRE LRNRNFGKHL REAEADKRES QLLLNRIRTW QKTHQGENNG LANSIRDSLN421EYEAKLSDLR ARLQEAAAQA KQANGLNQEN ERALGAIQRQ VKEINSLQSD FTKYLTTADS481SLLQTNIALQ LMEKSQKEYE KLAASLNEAR QELSDKVREL SRSAGKTSLV EEAEKHARSL541QELAKQLEEI KRNASGDELV RCAVDAATAY ENILNAIKAA EDAANRAASA SESALQTVIK601EDLPRKAKTL SSNSDKLLNE AKMTQKKLKQ EVSPALNNLQ QTLNIVTVQK EVIDTNLTTL661RDGLHGIQRG DIDAMISSAK SMVRKANDIT DEVLDGLNPI QTDVERIKDT YGRTQNEDFK721KALTDADNSV NKLTNKLPDL WRKIESINQQ LLPLGNISDN MDRIRELIQQ ARDAASKVAV781PMRFNGKSGV EVRLPNDLED LKGYTSLSLF LQRPNSRENG GTENMFVMYL GNKDASRDYI841GMAVVDGQLT CVYNLGDREA ELQVDQILTK SETKEAVMDR VKFQRIYQFA RLNYTKGATS901SKPETPGVYD MDGRNSNTLL NLDPENVVFY VGGYPPDFKL PSRLSFPPYK GCIELDDLNE961NVLSLYNFKK TFNLNTTEVE PCRRRKEESD KNYFEGTGYA RVPTQPHAPI PTFGQTIQTT1021VDRGLLFFAE NGDRFISLNI EDGKLMVRYK LNSELPKERG VGDAINNGRD HSIQIKIGKL1081QKRMWINVDV QNTIIDGEVF DFSTYYLGGI PIAIRERFNI STPAFRGCMK NLKKTSGVVR1141LNDTVGVTKK CSEDWKLVRS ASFSRGGQLS FTDLGLPPTD HLQASFGFQT FQPSGILLDH1201QTWTRNLQVT LEDGYIELST SDSGSPIFKS PQTYMDGLLH YVSVISDNSG LRLLIDDQLL1261RNSKRLKHIS SSRQSLRLGG SNFEGCISNV FVQRLSLSPE VLDLTSNSLK RDVSLGGCSL1321NKPPFLMLLK GSTRFNKTKT FRINQLLQDT PVASPRSVKV WQDACSPLPK TQANHGALQF1381GDIPTSHLLF KLPQELLKPR SQFAVDMQTT SSRGLVFHTG TKNSFMALYL SKGRLVFALG1441TDGKKLRIKS KEKCNDGKWH TVVFGHDGEK GRLVVDGLRA REGSLPGNST ISIRAPVYLG1501SPPSGKPKSL PTNSFVGCLK NFQLDSKPLY TPSSSFGVSS CLGGPLEKGI YFSEEGGHVV1561LAHSVLLGPE FKLVFSIRPR SLTGILIHIG SQPGKHLCVY LEAGKVTASM DSGAGGTSTS1621VTPKQSLCDG QWHSVAVTIK QHILHLELDT DSSYTAGQIP FPPASTQEPL HLGGAPANLT1681TLRIPVWKSF FGCLRNIHVN HIPVPVTEAL EVQGPVSLNG CPDQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1724 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.