LSBio
Human LAMA3 / Laminin Alpha 3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human LAMA3 / Laminin Alpha 3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all LAMA3 antibodies →
$220.00each
Catalog Number: LS-G77068-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human LAMA3 (NM_000227) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BM600 | Epiligrin 170 kDa subunit | E170 | Kalinin-165kDa | LAMA3 | Lama3a | Laminin Alpha 3 | Laminin alpha-3 chain | Laminin-5 alpha 3 chain | Nicein-150kDa | Laminin | alpha 3 | LOCS | Nicein 150kD subunit | BM600 150kD subunit | BM600-150kDa | Epiligrin | Epiligrin alpha 3 subunit | Epiligrin subunit alpha | Kalinin 165kD subunit | Kalinin subunit alpha | Laminin subunit alpha-3 | Laminin-5 subunit alpha | Laminin-6 subunit alpha | Laminin-7 subunit alpha | LAMNA | Nicein subunit alpha
Origin Species
Human
NCBI GenBank
NM_000227
SwissProt
Q16787

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
190.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPPAVRRSAC SMGWLWIFGA ALGQCLGYSS QQQRVPFLQP PGQSQLQASY VEFRPSQGCS
61PGYYRDHKGL YTGRCVPCNC NGHSNQCQDG SGICVNCQHN TAGEHCERCQ EGYYGNAVHG
121SCRACPCPHT NSFATGCVVN GGDVRCSCKA GYTGTQCERC APGYFGNPQK FGGSCQPCSC
181NSNGQLGSCH PLTGDCINQE PKDSSPAEEC DDCDSCVMTL LNDLATMGEQ LRLVKSQLQG
241LSASAGLLEQ MRHMETQAKD LRNQLLNYRS AISNHGSKIE GLERELTDLN QEFETLQEKA
301QVNSRKAQTL NNNVNRATQS AKELDVKIKN VIRNVHILLK QISGTDGEGN NVPSGDFSRE
361WAEAQRMMRE LRNRNFGKHL REAEADKRES QLLLNRIRTW QKTHQGENNG LANSIRDSLN
421EYEAKLSDLR ARLQEAAAQA KQANGLNQEN ERALGAIQRQ VKEINSLQSD FTKYLTTADS
481SLLQTNIALQ LMEKSQKEYE KLAASLNEAR QELSDKVREL SRSAGKTSLV EEAEKHARSL
541QELAKQLEEI KRNASGDELV RCAVDAATAY ENILNAIKAA EDAANRAASA SESALQTVIK
601EDLPRKAKTL SSNSDKLLNE AKMTQKKLKQ EVSPALNNLQ QTLNIVTVQK EVIDTNLTTL
661RDGLHGIQRG DIDAMISSAK SMVRKANDIT DEVLDGLNPI QTDVERIKDT YGRTQNEDFK
721KALTDADNSV NKLTNKLPDL WRKIESINQQ LLPLGNISDN MDRIRELIQQ ARDAASKVAV
781PMRFNGKSGV EVRLPNDLED LKGYTSLSLF LQRPNSRENG GTENMFVMYL GNKDASRDYI
841GMAVVDGQLT CVYNLGDREA ELQVDQILTK SETKEAVMDR VKFQRIYQFA RLNYTKGATS
901SKPETPGVYD MDGRNSNTLL NLDPENVVFY VGGYPPDFKL PSRLSFPPYK GCIELDDLNE
961NVLSLYNFKK TFNLNTTEVE PCRRRKEESD KNYFEGTGYA RVPTQPHAPI PTFGQTIQTT
1021VDRGLLFFAE NGDRFISLNI EDGKLMVRYK LNSELPKERG VGDAINNGRD HSIQIKIGKL
1081QKRMWINVDV QNTIIDGEVF DFSTYYLGGI PIAIRERFNI STPAFRGCMK NLKKTSGVVR
1141LNDTVGVTKK CSEDWKLVRS ASFSRGGQLS FTDLGLPPTD HLQASFGFQT FQPSGILLDH
1201QTWTRNLQVT LEDGYIELST SDSGSPIFKS PQTYMDGLLH YVSVISDNSG LRLLIDDQLL
1261RNSKRLKHIS SSRQSLRLGG SNFEGCISNV FVQRLSLSPE VLDLTSNSLK RDVSLGGCSL
1321NKPPFLMLLK GSTRFNKTKT FRINQLLQDT PVASPRSVKV WQDACSPLPK TQANHGALQF
1381GDIPTSHLLF KLPQELLKPR SQFAVDMQTT SSRGLVFHTG TKNSFMALYL SKGRLVFALG
1441TDGKKLRIKS KEKCNDGKWH TVVFGHDGEK GRLVVDGLRA REGSLPGNST ISIRAPVYLG
1501SPPSGKPKSL PTNSFVGCLK NFQLDSKPLY TPSSSFGVSS CLGGPLEKGI YFSEEGGHVV
1561LAHSVLLGPE FKLVFSIRPR SLTGILIHIG SQPGKHLCVY LEAGKVTASM DSGAGGTSTS
1621VTPKQSLCDG QWHSVAVTIK QHILHLELDT DSSYTAGQIP FPPASTQEPL HLGGAPANLT
1681TLRIPVWKSF FGCLRNIHVN HIPVPVTEAL EVQGPVSLNG CPDQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1724 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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