Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT84 / Keratin 84 / KRTHB4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108453-100
Catalog Number: LS-G108453
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT84 (NM_033045), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hard keratin | type II | 4 | HB4 | K84 | Keratin 84 | Keratin | type II cuticular Hb4 | Hb-4 | KRT84 | KRTHB4 | Type II hair keratin Hb4 | hair | basic | Keratin-84 | Type II hair keratin 4 | Type-II keratin Kb24
- Origin Species
- Human
- SwissProt
- Q9NSB2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSCRSYRVSS GHRVGNFSSC SAMTPQNLNR FRANSVSCWS GPGFRGLGSF GSRSVITFGS61YSPRIAAVGS RPIHCGVRFG AGCGMGFGDG RGVGLGPRAD SCVGLGFGAG SGIGYGFGGP121GFGYRVGGVG VPAAPSITAV TVNKSLLTPL NLEIDPNAQR VKKDEKEQIK TLNNKFASFI181DKVRFLEQQN KLLETKWSFL QEQKCIRSNL EPLFESYITN LRRQLEVLVS DQARLQAERN241HLQDVLEGFK KKYEEEVVCR ANAENEFVAL KKDVDAAFMN KSDLEANVDT LTQEIDFLKT301LYMEEIQLLQ SHISETSVIV KMDNSRDLNL DGIIAEVKAQ YEEVARRSRA DAEAWYQTKY361EEMQVTAGQH CDNLRNIRNE INELTRLIQR LKAEIEHAKA QRAKLEAAVA EAEQQGEATL421SDAKCKLADL ECALQQAKQD MARQLCEYQE LMNAKLGLDI EIATYRRLLE GEESRLCEGV481GPVNISVSSS RGGLVCGPEP LVAGSTLSRG GVTFSGSSSV CATSGVLASC GPSLGGARVA541PATGDLLSTG TRSGSMLISE ACVPSVPCPL PTQGGFSSCS GGRSSSVRFV STTTSCRTKYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
600 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.