LSBio
Human KRT84 / Keratin 84 / KRTHB4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KRT84 / Keratin 84 / KRTHB4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KRT84 antibodies →
$153.00each
Catalog Number: LS-G92457-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KRT84 (NM_033045) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Hard keratin | type II | 4 | HB4 | K84 | Keratin 84 | Keratin | type II cuticular Hb4 | Hb-4 | KRT84 | KRTHB4 | Type II hair keratin Hb4 | hair | basic | Keratin-84 | Type II hair keratin 4 | Type-II keratin Kb24
Origin Species
Human
SwissProt
Q9NSB2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSCRSYRVSS GHRVGNFSSC SAMTPQNLNR FRANSVSCWS GPGFRGLGSF GSRSVITFGS
61YSPRIAAVGS RPIHCGVRFG AGCGMGFGDG RGVGLGPRAD SCVGLGFGAG SGIGYGFGGP
121GFGYRVGGVG VPAAPSITAV TVNKSLLTPL NLEIDPNAQR VKKDEKEQIK TLNNKFASFI
181DKVRFLEQQN KLLETKWSFL QEQKCIRSNL EPLFESYITN LRRQLEVLVS DQARLQAERN
241HLQDVLEGFK KKYEEEVVCR ANAENEFVAL KKDVDAAFMN KSDLEANVDT LTQEIDFLKT
301LYMEEIQLLQ SHISETSVIV KMDNSRDLNL DGIIAEVKAQ YEEVARRSRA DAEAWYQTKY
361EEMQVTAGQH CDNLRNIRNE INELTRLIQR LKAEIEHAKA QRAKLEAAVA EAEQQGEATL
421SDAKCKLADL ECALQQAKQD MARQLCEYQE LMNAKLGLDI EIATYRRLLE GEESRLCEGV
481GPVNISVSSS RGGLVCGPEP LVAGSTLSRG GVTFSGSSSV CATSGVLASC GPSLGGARVA
541PATGDLLSTG TRSGSMLISE ACVPSVPCPL PTQGGFSSCS GGRSSSVRFV STTTSCRTKY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

600 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.