Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT76 / Keratin 76 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113045-100
Catalog Number: LS-G113045
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cytokeratin 2(KRT76) (NM_015848), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 65 kDa cytokeratin | CK-3 | Cytokeratin 2e | Cytokeratin-3 | Ck 2e | Cytokeratin 3 | Keratin-3 | Keratin 3 | Krt2e | KRT3 | Type-II keratin Kb3 | CK3 | K3
- Origin Species
- Human
- NCBI GenBank
- NM_015848
- SwissProt
- Q01546
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNRQVCKKSF SGRSQGFSGR SAVVSGSSRM SCVARSGGAG GGACGFRSGA GSFGSRSLYN61LGSNKSISIS VAAGSSRAGG FGGGRSSCGF AGGYGGGFGG SYGGGFGGGR GVGSGFGGAG121GFGGAGGFGG PGVFGGPGSF GGPGGFGPGG FPGGIQEVIV NQSLLQPLNV EIDPQIGQVK181AQEREQIKTL NNKFASFIDK VRFLEQQNKV LETKWELLQQ QTTGSGPSSL EPCFESYISF241LCKQLDSLLG ERGNLEGELK SMQDLVEDFK KKYEDEINKR TAAENEFVGL KKDVDAAFMN301KVELQAKVDS LTDEVSFLRT LYEMELSQMQ SHASDTSVVL SMDNNRCLDL GSIIAEVRAQ361YEEIAQRSKS EAEALYQTKL GELQTTAGRH GDDLRNTKSE IMELNRMIQR LRAEIENVKK421QNANLQTAIA EAEQRGEMAL KDANAKLQDL QTALQKAKDD LARLLRDYQE LMNVKLALDV481EIATYRKLLE GEECRMSGEC QSAVCISVVS NVTSTSGSSG SSRGVFGGVS GSGSGGYKGG541SSSSSSSGYG VSGGSGSGYG GVSSGSTGGR GSSGSYQSSS SGSRLGGAGS ISVSHSGMGS601SSGSIQTSGG SGYKSGGGGS TSIRFSQTTS SSQHSSTKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
638 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.