KRT76 / Keratin 76 Antibody
Catalog Number: LS-C831370
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human KRT76 / Keratin 76 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G76986-20
Catalog Number: LS-G76986
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Cytokeratin 2(KRT76) (NM_015848) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 65 kDa cytokeratin | CK-3 | Cytokeratin 2e | Cytokeratin-3 | Ck 2e | Cytokeratin 3 | Keratin-3 | Keratin 3 | Krt2e | KRT3 | Type-II keratin Kb3 | CK3 | K3
- Origin Species
- Human
- NCBI GenBank
- NM_015848
- SwissProt
- Q01546
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNRQVCKKSF SGRSQGFSGR SAVVSGSSRM SCVARSGGAG GGACGFRSGA GSFGSRSLYN61LGSNKSISIS VAAGSSRAGG FGGGRSSCGF AGGYGGGFGG SYGGGFGGGR GVGSGFGGAG121GFGGAGGFGG PGVFGGPGSF GGPGGFGPGG FPGGIQEVIV NQSLLQPLNV EIDPQIGQVK181AQEREQIKTL NNKFASFIDK VRFLEQQNKV LETKWELLQQ QTTGSGPSSL EPCFESYISF241LCKQLDSLLG ERGNLEGELK SMQDLVEDFK KKYEDEINKR TAAENEFVGL KKDVDAAFMN301KVELQAKVDS LTDEVSFLRT LYEMELSQMQ SHASDTSVVL SMDNNRCLDL GSIIAEVRAQ361YEEIAQRSKS EAEALYQTKL GELQTTAGRH GDDLRNTKSE IMELNRMIQR LRAEIENVKK421QNANLQTAIA EAEQRGEMAL KDANAKLQDL QTALQKAKDD LARLLRDYQE LMNVKLALDV481EIATYRKLLE GEECRMSGEC QSAVCISVVS NVTSTSGSSG SSRGVFGGVS GSGSGGYKGG541SSSSSSSGYG VSGGSGSGYG GVSSGSTGGR GSSGSYQSSS SGSRLGGAGS ISVSHSGMGS601SSGSIQTSGG SGYKSGGGGS TSIRFSQTTS SSQHSSTKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
638 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
