Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KRT75 / Keratin 75 / K6HF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116482-100
Catalog Number: LS-G116482
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KRT75 (NM_004693), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cytokeratin type II | KB18 | Keratin-6 hair follicle | K75 | PFB | Keratin 6 isoform k6f | Keratin 75 | KRT75 | Type II keratin-K6hf | Type-II keratin Kb18 | Type II keratin-18 | CK-75 | Cytokeratin-75 | HK6hf | K6HF | Keratin-75 | Krt6f
- Origin Species
- Human
- NCBI GenBank
- NM_004693
- SwissProt
- O95678
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSRQSSITFQ SGSRRGFSTT SAITPAAGRS RFSSVSVARS AAGSGGLGRI SSAGASFGSR61SLYNLGGAKR VSINGCGSSC RSGFGGRASN RFGVNSGFGY GGGVGGGFSG PSFPVCPPGG121IQEVTVNQSL LTPLHLQIDP TIQRVRAEER EQIKTLNNKF ASFIDKVRFL EQQNKVLETK181WALLQEQGSR TVRQNLEPLF DSYTSELRRQ LESITTERGR LEAELRNMQD VVEDFKVRYE241DEINKRTAAE NEFVALKKDV DAAYMNKVEL EAKVKSLPEE INFIHSVFDA ELSQLQTQVG301DTSVVLSMDN NRNLDLDSII AEVKAQYEDI ANRSRAEAES WYQTKYEELQ VTAGRHGDDL361RNTKQEISEM NRMIQRLRAE IDSVKKQCSS LQTAIADAEQ RGELALKDAR AKLVDLEEAL421QKAKQDMARL LREYQELMNI KLALDVEIAT YRKLLEGEEC RLSGEGVSPV NISVVTSTLS481SGYGSGSSIG GGNLGLGGGS GYSFTTSGGH SLGAGLGGSG FSATSNRGLG GSGSSVKFVS541TTSSSQKSYT HTRTRPLEQKLISEEDLAANDILDYKDDDDKV
551 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.