LSBio
Human KRT75 / Keratin 75 / K6HF Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KRT75 / Keratin 75 / K6HF Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KRT75 antibodies →
$220.00each
Catalog Number: LS-G88936-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KRT75 (NM_004693) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cytokeratin type II | KB18 | Keratin-6 hair follicle | K75 | PFB | Keratin 6 isoform k6f | Keratin 75 | KRT75 | Type II keratin-K6hf | Type-II keratin Kb18 | Type II keratin-18 | CK-75 | Cytokeratin-75 | HK6hf | K6HF | Keratin-75 | Krt6f
Origin Species
Human
NCBI GenBank
NM_004693
SwissProt
O95678

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MSRQSSITFQ SGSRRGFSTT SAITPAAGRS RFSSVSVARS AAGSGGLGRI SSAGASFGSR
61SLYNLGGAKR VSINGCGSSC RSGFGGRASN RFGVNSGFGY GGGVGGGFSG PSFPVCPPGG
121IQEVTVNQSL LTPLHLQIDP TIQRVRAEER EQIKTLNNKF ASFIDKVRFL EQQNKVLETK
181WALLQEQGSR TVRQNLEPLF DSYTSELRRQ LESITTERGR LEAELRNMQD VVEDFKVRYE
241DEINKRTAAE NEFVALKKDV DAAYMNKVEL EAKVKSLPEE INFIHSVFDA ELSQLQTQVG
301DTSVVLSMDN NRNLDLDSII AEVKAQYEDI ANRSRAEAES WYQTKYEELQ VTAGRHGDDL
361RNTKQEISEM NRMIQRLRAE IDSVKKQCSS LQTAIADAEQ RGELALKDAR AKLVDLEEAL
421QKAKQDMARL LREYQELMNI KLALDVEIAT YRKLLEGEEC RLSGEGVSPV NISVVTSTLS
481SGYGSGSSIG GGNLGLGGGS GYSFTTSGGH SLGAGLGGSG FSATSNRGLG GSGSSVKFVS
541TTSSSQKSYT H
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

551 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.