Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KREMEN1 / KREMEN-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120624-100
Catalog Number: LS-G120624
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KREMEN1 (NM_001039571), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Dickkopf receptor | KREMEN | Kremen protein 1 | KREMEN1 | KRM1 | KREMEM1
- Origin Species
- Human
- NCBI GenBank
- NM_001039570
- SwissProt
- Q96MU8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.18 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPPAARLAL LSAAALTLAA RPAPSPGLGP GPECFTANGA DYRGTQNWTA LQGGKPCLFW61NETFQHPYNT LKYPNGEGGL GEHNYCRNPD GDVSPWCYVA EHEDGVYWKY CEIPACQMPG121NLGCYKDHGN PPPLTGTSKT SNKLTIQTCI SFCRSQRFKF AGMESGYACF CGNNPDYWKY181GEAASTECNS VCFGDHTQPC GGDGRIILFD TLVGACGGNY SAMSSVVYSP DFPDTYATGR241VCYWTIRVPG ASHIHFSFPL FDIRDSADMV ELLDGYTHRV LARFHGRSRP PLSFNVSLDF301VILYFFSDRI NQAQGFAVLY QAVKEELPQE RPAVNQTVAE VITEQANLSV SAARSSKVLY361VITTSPSHPP QTVPGWTVYG LATLLILTVT AIVAKILLHV TFKSHRVPAS GDLRDCHQPG421TSGEIWSIFY KPSTSISIFK KKLKGQSQQD DRNPLVSDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
458 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.