LSBio
Human KREMEN1 / KREMEN-1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KREMEN1 / KREMEN-1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120623-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KREMEN1 (NM_001039570), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Dickkopf receptor | KREMEN | Kremen protein 1 | KREMEN1 | KRM1 | KREMEM1
Origin Species
Human
NCBI GenBank
NM_001039570
SwissProt
Q96MU8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPPAARLAL LSAAALTLAA RPAPSPGLGP GPECFTANGA DYRGTQNWTA LQGGKPCLFW
61NETFQHPYNT LKYPNGEGGL GEHNYCRNPD GDVSPWCYVA EHEDGVYWKY CEIPACQMPG
121NLGCYKDHGN PPPLTGTSKT SNKLTIQTCI SFCRSQRFKF AGMESGYACF CGNNPDYWKY
181GEAASTECNS VCFGDHTQPC GGDGRIILFD TLVGACGGNY SAMSSVVYSP DFPDTYATGR
241VCYWTIRVPG ASHIHFSFPL FDIRDSADMV ELLDGYTHRV LARFHGRSRP PLSFNVSLDF
301VILYFFSDRI NQAQGFAVLY QAVKEELPQE RPAVNQTVAE VITEQANLSV SAARSSKVLY
361VITTSPSHPP QTVPGSNSWA PPMGAGSHRV EGWTVYGLAT LLILTVTAIV AKILLHVTFK
421SHRVPASGDL RDCHQPGTSG EIWSIFYKPS TSISIFKKKL KGQSQQDDRN PLVSD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

475 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.