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KLHL13 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL13 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G130114-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL13 (NM_001168301), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BKLHD2 | Kelch-like 13 (Drosophila) | Kelch-like protein 13 | KIAA1309 | KLHL13
Origin Species
Human
NCBI GenBank
AB037730
SwissProt
Q9P2N7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDHLHRGELV AAILRNRSLV EEEDQHMKLS LGGSEMGLSS HLQSSKAGPT RIFTSNTHSS
61VVLQGFDQLR LEGLLCDVTL MPGDTDDAFP VHRVMMASAS DYFKAMFTGG MKEQDLMCIK
121LHGVSKVGLR KIIDFIYTAK LSLNMDNLQD TLEAASFLQI LPVLDFCKVF LISGVTLDNC
181VEVGRIANTY NLTEVDKYVN SFVLKNFPAL LSTGEFLKLP FERLAFVLSS NSLKHCTELE
241LFKATCRWLR LEEPRMDFAA KLMKNIRFPL MTPQELINYV QTVDFMRTDN TCVNLLLEAS
301NYQMMPYMQP VMQSDRTAIR SDTTHLVTLG GVLRQQLVVS KELRMYDEKA HEWKSLAPMD
361APRYQHGIAV IGNFLYVVGG QSNYDTKGKT AVDTVFRFDP RYNKWMQVAS LNEKRTFFHL
421SALKGYLYAV GGRNAAGELP TVECYNPRTN EWTYVAKMSE PHYGHAGTVY GGVMYISGGI
481THDTFQKELM CFDPDTDKWI QKAPMTTVRG LHCMCTVGER LYVIGGNHFR GTSDYDDVLS
541CEYYSPILDQ WTPIAAMLRG QSDVGVAVFE NKIYVVGGYS WNNRCMVEIV QKYDPDKDEW
601HKVFDLPESL GGIRACTLTV FPPEETTPSP SRESPLSAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

639 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.