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KLHL13 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL13 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108227-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL13 (NM_033495), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BKLHD2 | Kelch-like 13 (Drosophila) | Kelch-like protein 13 | KIAA1309 | KLHL13
Origin Species
Human
NCBI GenBank
AB037730
SwissProt
Q9P2N7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPLKWKTSSP AIWKFPVPVL KTSRSTPLSP AYISLVEEED QHMKLSLGGS EMGLSSHLQS
61SKAGPTRIFT SNTHSSVVLQ GFDQLRLEGL LCDVTLMPGD TDDAFPVHRV MMASASDYFK
121AMFTGGMKEQ DLMCIKLHGV SKVGLRKIID FIYTAKLSLN MDNLQDTLEA ASFLQILPVL
181DFCKVFLISG VTLDNCVEVG RIANTYNLTE VDKYVNSFVL KNFPALLSTG EFLKLPFERL
241AFVLSSNSLK HCTELELFKA TCRWLRLEEP RMDFAAKLMK NIRFPLMTPQ ELINYVQTVD
301FMRTDNTCVN LLLEASNYQM MPYMQPVMQS DRTAIRSDTT HLVTLGGVLR QQLVVSKELR
361MYDEKAHEWK SLAPMDAPRY QHGIAVIGNF LYVVGGQSNY DTKGKTAVDT VFRFDPRYNK
421WMQVASLNEK RTFFHLSALK GYLYAVGGRN AAGELPTVEC YNPRTNEWTY VAKMSEPHYG
481HAGTVYGGVM YISGGITHDT FQKELMCFDP DTDKWIQKAP MTTVRGLHCM CTVGERLYVI
541GGNHFRGTSD YDDVLSCEYY SPILDQWTPI AAMLRGQSDV GVAVFENKIY VVGGYSWNNR
601CMVEIVQKYD PDKDEWHKVF DLPESLGGIR ACTLTVFPPE ETTPSPSRES PLSAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

655 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.