Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KLF5 / BTEB2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118427-100
Catalog Number: LS-G118427
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLF5 (NM_001730), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BTE-binding protein 2 | BTEB2 | Colon kruppel-like factor | Colon krueppel-like factor | GC box binding protein 2 | IKLF | Gc box-binding protein bteb2 | GC-box-binding protein 2 | KLF5 | Transcription factor BTEB2 | Krueppel-like factor 5
- Origin Species
- Human
- NCBI GenBank
- NM_001730
- SwissProt
- Q13887
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MATRVLSMSA RLGPVPQPPA PQDEPVFAQL KPVLGAANPA RDAALFPGEE LKHAHHRPQA61QPAPAQAPQP AQPPATGPRL PPEDLVQTRC EMEKYLTPQL PPVPIIPEHK KYRRDSASVV121DQFFTDTEGL PYSINMNVFL PDITHLRTGL YKSQRPCVTH IKTEPVAIFS HQSETTAPPP181APTQALPEFT SIFSSHQTAA PEVNNIFIKQ ELPTPDLHLS VPTQQGHLYQ LLNTPDLDMP241SSTNQTAAMD TLNVSMSAAM AGLNTHTSAV PQTAVKQFQG MPPCTYTMPS QFLPQQATYF301PPSPPSSEPG SPDRQAEMLQ NLTPPPSYAA TIASKLAIHN PNLPTTLPVN SQNIQPVRYN361RRSNPDLEKR RIHYCDYPGC TKVYTKSSHL KAHLRTHTGE KPYKCTWEGC DWRFARSDEL421TRHYRKHTGA KPFQCGVCNR SFSRSDHLAL HMKRHQNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
457 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.