LSBio
Human KLF5 / BTEB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KLF5 / BTEB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KLF5 antibodies →
$153.00each
Catalog Number: LS-G76115-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KLF5 (NM_001730) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BTE-binding protein 2 | BTEB2 | Colon kruppel-like factor | Colon krueppel-like factor | GC box binding protein 2 | IKLF | Gc box-binding protein bteb2 | GC-box-binding protein 2 | KLF5 | Transcription factor BTEB2 | Krueppel-like factor 5
Origin Species
Human
NCBI GenBank
NM_001730
SwissProt
Q13887

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MATRVLSMSA RLGPVPQPPA PQDEPVFAQL KPVLGAANPA RDAALFPGEE LKHAHHRPQA
61QPAPAQAPQP AQPPATGPRL PPEDLVQTRC EMEKYLTPQL PPVPIIPEHK KYRRDSASVV
121DQFFTDTEGL PYSINMNVFL PDITHLRTGL YKSQRPCVTH IKTEPVAIFS HQSETTAPPP
181APTQALPEFT SIFSSHQTAA PEVNNIFIKQ ELPTPDLHLS VPTQQGHLYQ LLNTPDLDMP
241SSTNQTAAMD TLNVSMSAAM AGLNTHTSAV PQTAVKQFQG MPPCTYTMPS QFLPQQATYF
301PPSPPSSEPG SPDRQAEMLQ NLTPPPSYAA TIASKLAIHN PNLPTTLPVN SQNIQPVRYN
361RRSNPDLEKR RIHYCDYPGC TKVYTKSSHL KAHLRTHTGE KPYKCTWEGC DWRFARSDEL
421TRHYRKHTGA KPFQCGVCNR SFSRSDHLAL HMKRHQN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

457 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.