Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIZ / PLK1S1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111728-100
Catalog Number: LS-G111728
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C20orf19(KIZ) (NM_018474), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Centrosomal protein kizuna | Kizuna | Kiz | NCRNA00153 | Non-protein coding RNA 153 | Polo-like kinase 1 substrate 1 | C20orf19 | HT013 | PLK1S1
- Origin Species
- Human
- NCBI GenBank
- NM_018474
- SwissProt
- Q2M2Z5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSRTLASAVP LSSPDYYERL GQLQHGLRDS EKKRLDLEKK LYEYNQSDTC RVKLKYVKLK61NYLKEICESE KKAHTRNQEY LKRFERVQAH VVHFTTNTEK LQKLKLEYET QIKKMLCSKD121SLGLKEELTD EDREKVAVQE GINSGTAMSR GLYQPATIFM GRQMSAILSM RDFSTEHKSP181QPTKNFSIPD PHSHRQTAQS SNVTDSCVVQ TSNDTQCLNK SDNIDGKASL QIGEKTPVTA241SVLSEEEQTH CLEIGSNTRH GKSNLSEGKK SAELNSPLRE RLSPENRTTD LKCDSSSGSE301GEILTREHIE VEEKRASPPV SPIPVSEYCE SENKWSQEKH SPWEGVSDHL AHREPKSQKP361FRKMQEEEEE SWSTSSDLTI SISEDDLILE SPEPQPNPGG KMEGEDGIEA LKLIHAEQER421VALSTEKNCI LQTLSSPDSE KESSTNAPTR EPGQTPDSDV PRAQVGQHVA TLKEHDNSVK481EEATALLRKA LTEECGRRSA IHSSESSCSL PSILNDNSGI KEAKPAVWLN SVPTREQEVS541SGCGDKSKKE NVAADIPITE TEAYQLLKKA TLQDNTNQTE NRFQKTDASV SHLSGLNIGS601GAFETKTANK IASEASFSSS EGSPLSRHEN KKKPVINLKS NALWDESDDS NSEIEAALRP661RNHNTDDSDD FYDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
673 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.