LSBio
Human KIZ / PLK1S1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KIZ / PLK1S1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KIZ antibodies →
$153.00each
Catalog Number: LS-G82858-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C20orf19(KIZ) (NM_018474) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Centrosomal protein kizuna | Kizuna | Kiz | NCRNA00153 | Non-protein coding RNA 153 | Polo-like kinase 1 substrate 1 | C20orf19 | HT013 | PLK1S1
Origin Species
Human
NCBI GenBank
NM_018474
SwissProt
Q2M2Z5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSRTLASAVP LSSPDYYERL GQLQHGLRDS EKKRLDLEKK LYEYNQSDTC RVKLKYVKLK
61NYLKEICESE KKAHTRNQEY LKRFERVQAH VVHFTTNTEK LQKLKLEYET QIKKMLCSKD
121SLGLKEELTD EDREKVAVQE GINSGTAMSR GLYQPATIFM GRQMSAILSM RDFSTEHKSP
181QPTKNFSIPD PHSHRQTAQS SNVTDSCVVQ TSNDTQCLNK SDNIDGKASL QIGEKTPVTA
241SVLSEEEQTH CLEIGSNTRH GKSNLSEGKK SAELNSPLRE RLSPENRTTD LKCDSSSGSE
301GEILTREHIE VEEKRASPPV SPIPVSEYCE SENKWSQEKH SPWEGVSDHL AHREPKSQKP
361FRKMQEEEEE SWSTSSDLTI SISEDDLILE SPEPQPNPGG KMEGEDGIEA LKLIHAEQER
421VALSTEKNCI LQTLSSPDSE KESSTNAPTR EPGQTPDSDV PRAQVGQHVA TLKEHDNSVK
481EEATALLRKA LTEECGRRSA IHSSESSCSL PSILNDNSGI KEAKPAVWLN SVPTREQEVS
541SGCGDKSKKE NVAADIPITE TEAYQLLKKA TLQDNTNQTE NRFQKTDASV SHLSGLNIGS
601GAFETKTANK IASEASFSSS EGSPLSRHEN KKKPVINLKS NALWDESDDS NSEIEAALRP
661RNHNTDDSDD FYD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

673 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.