Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIF1B / CMT2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103965-100
Catalog Number: LS-G103965
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF1B (NM_183416), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CMT2 | CMT2A | HMSNII | KIAA0591 | KIAA1448 | Kinesin family member 1B | Kinesin-like protein KIF1B | KLP | NBLST1 | CMT2A1 | KIF1B
- Origin Species
- Human
- NCBI GenBank
- AB011163
- SwissProt
- O60333
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 130.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGASVKVAV RVRPFNSRET SKESKCIIQM QGNSTSIINP KNPKEAPKSF SFDYSYWSHT61SPEDPCFASQ NRVYNDIGKE MLLHAFEGYN VCIFAYGQTG AGKSYTMMGK QEESQAGIIP121QLCEELFEKI NDNCNEEMSY SVEVSYMEIY CERVRDLLNP KNKGNLRVRE HPLLGPYVED181LSKLAVTSYT DIADLMDAGN KARTVAATNM NETSSRSHAV FTIVFTQKKH DNETNLSTEK241VSKISLVDLA GSERADSTGA KGTRLKEGAN INKSLTTLGK VISALAEVSK KKKKTDFIPY301RDSVLTWLLR ENLGGNSRTA MVAALSPADI NYDETLSTLR YADRAKQIKC NAVINEDPNA361KLVRELKEEV TRLKDLLRAQ GLGDIIDTSM GSLTSSPSSC SLSSQVGLTS VTSIQERIMS421TPGGEEAIER LKESEKIIAE LNETWEEKLR KTEAIRMERE ALLAEMGVAI REDGGTLGVF481SPKKTPHLVN LNEDPLMSEC LLYYIKDGIT RVGQADAERR QDIVLSGAHI KEEHCIFRSE541RSNSGEVIVT LEPCERSETY VNGKRVSQPV QLRSGNRIIM GKNHVFRFNH PEQARAEREK601TPSAETPSEP VDWTFAQREL LEKQGIDMKQ EMEKRLQEME ILYKKEKEEA DLLLEQQRLD661ADSDSGDDSD KRSCEESWKL ITSLREKLPP SKLQTIVKKC GLPSSGKKRE PIKMYQIPQR721RRLSKDSKWV TISDLKIQAV KEICYEVALN DFRHSRQEIE ALAIVKMKEL CAMYGKKDPN781ERDSWRAVAR DVWDTVGVGD EKIEDVMATG KGSTDVDDLK VHIDKLEDIL QEVKKQNNMK841DEEIKVLRNK MLKMEKVLPL IGSQEQKSPG SHKAKEPVGA GVSSTSENNV SKGDNGELAK901EERVSQLMNG DPAFRRGRLR WMRQEQIRFK NLQQQEITKQ LRRQNVPHRF IPPENRKPRF961PFKSNPKHRN SWSPGTHIII TEDEVIELRI PKDDEARKGN KEESQEKGGK GAFKDPQFPW1021GSQGMRSQDH IQVSKQHINN QQQPPQLRWR SNSLNNGQPK STRCQASASA ESLNSHSGHP1081TADVQTFQAK RHIHQHRQSY CNYNTGGQLE GNAATSYQKQ TDKPSHCSQF VTPPRMRRQF1141SAPNLKAGRE TTVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1153 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.