LSBio
Human KIF1B / CMT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KIF1B / CMT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KIF1B antibodies →
$220.00each
Catalog Number: LS-G79966-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KIF1B (NM_183416) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CMT2 | CMT2A | HMSNII | KIAA0591 | KIAA1448 | Kinesin family member 1B | Kinesin-like protein KIF1B | KLP | NBLST1 | CMT2A1 | KIF1B
Origin Species
Human
NCBI GenBank
AB011163
SwissProt
O60333

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
130.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSGASVKVAV RVRPFNSRET SKESKCIIQM QGNSTSIINP KNPKEAPKSF SFDYSYWSHT
61SPEDPCFASQ NRVYNDIGKE MLLHAFEGYN VCIFAYGQTG AGKSYTMMGK QEESQAGIIP
121QLCEELFEKI NDNCNEEMSY SVEVSYMEIY CERVRDLLNP KNKGNLRVRE HPLLGPYVED
181LSKLAVTSYT DIADLMDAGN KARTVAATNM NETSSRSHAV FTIVFTQKKH DNETNLSTEK
241VSKISLVDLA GSERADSTGA KGTRLKEGAN INKSLTTLGK VISALAEVSK KKKKTDFIPY
301RDSVLTWLLR ENLGGNSRTA MVAALSPADI NYDETLSTLR YADRAKQIKC NAVINEDPNA
361KLVRELKEEV TRLKDLLRAQ GLGDIIDTSM GSLTSSPSSC SLSSQVGLTS VTSIQERIMS
421TPGGEEAIER LKESEKIIAE LNETWEEKLR KTEAIRMERE ALLAEMGVAI REDGGTLGVF
481SPKKTPHLVN LNEDPLMSEC LLYYIKDGIT RVGQADAERR QDIVLSGAHI KEEHCIFRSE
541RSNSGEVIVT LEPCERSETY VNGKRVSQPV QLRSGNRIIM GKNHVFRFNH PEQARAEREK
601TPSAETPSEP VDWTFAQREL LEKQGIDMKQ EMEKRLQEME ILYKKEKEEA DLLLEQQRLD
661ADSDSGDDSD KRSCEESWKL ITSLREKLPP SKLQTIVKKC GLPSSGKKRE PIKMYQIPQR
721RRLSKDSKWV TISDLKIQAV KEICYEVALN DFRHSRQEIE ALAIVKMKEL CAMYGKKDPN
781ERDSWRAVAR DVWDTVGVGD EKIEDVMATG KGSTDVDDLK VHIDKLEDIL QEVKKQNNMK
841DEEIKVLRNK MLKMEKVLPL IGSQEQKSPG SHKAKEPVGA GVSSTSENNV SKGDNGELAK
901EERVSQLMNG DPAFRRGRLR WMRQEQIRFK NLQQQEITKQ LRRQNVPHRF IPPENRKPRF
961PFKSNPKHRN SWSPGTHIII TEDEVIELRI PKDDEARKGN KEESQEKGGK GAFKDPQFPW
1021GSQGMRSQDH IQVSKQHINN QQQPPQLRWR SNSLNNGQPK STRCQASASA ESLNSHSGHP
1081TADVQTFQAK RHIHQHRQSY CNYNTGGQLE GNAATSYQKQ TDKPSHCSQF VTPPRMRRQF
1141SAPNLKAGRE TTV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1153 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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