Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIDINS220 / ARMS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111048-100
Catalog Number: LS-G111048
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIDINS220 (NM_020738), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARMS | KIDINS220 | KIAA1250
- Origin Species
- Human
- NCBI GenBank
- AB033076
- SwissProt
- Q9ULH0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 196.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSVLISQSVI NYVEEENIPA LKALLEKCKD VDERNECGQT PLMIAAEQGN LEIVKELIKN61GANCNLEDLD NWTALISASK EGHVHIVEEL LKCGVNLEHR DMGGWTALMW ACYKGRTDVV121ELLLSHGANP SVTGLYSVYP IIWAAGRGHA DIVHLLLQNG AKVNCSDKYG TTPLVWAARK181GHLECVKHLL AMGADVDQEG ANSMTALIVA VKGGYTQSVK EILKRNPNVN LTDKDGNTAL241MIASKEGHTE IVQDLLDAGT YVNIPDRSGD TVLIGAVRGG HVEIVRALLQ KYADIDIRGQ301DNKTALYWAV EKGNATMVRD ILQCNPDTEI CTKDGETPLI KATKMRNIEV VELLLDKGAK361VSAVDKKGDT PLHIAIRGRS RKLAELLLRN PKDGRLLYRP NKAGETPYNI DCSHQKSILT421QIFGARHLSP TETDGDMLGY DLYSSALADI LSEPTMQPPI CVGLYAQWGS GKSFLLKKLE481DEMKTFAGQQ IEPLFQFSWL IVFLTLLLCG GLGLLFAFTV HPNLGIAVSL SFLALLYIFF541IVIYFGGRRE GESWNWAWVL STRLARHIGY LELLLKLMFV NPPELPEQTT KALPVRFLFT601DYNRLSSVGG ETSLAEMIAT LSDACEREFG FLATRLFRVF KTEDTQGKKK WKKTCCLPSF661VIFLFIIGCI ISGITLLAIF RVDPKHLTVN AVLISIASVV GLAFVLNCRT WWQVLDSLLN721SQRKRLHNAA SKLHKLKSEG FMKVLKCEVE LMARMAKTID SFTQNQTRLV VIIDGLDACE781QDKVLQMLDT VRVLFSKGPF IAIFASDPHI IIKAINQNLN SVLRDSNING HDYMRNIVHL841PVFLNSRGLS NARKFLVTSA TNGDVPCSDT TGIQEDADRR VSQNSLGEMT KLGSKTALNR901RDTYRRRQMQ RTITRQMSFD LTKLLVTEDW FSDISPQTMR RLLNIVSVTG RLLRANQISF961NWDRLASWIN LTEQWPYRTS WLILYLEETE GIPDQMTLKT IYERISKNIP TTKDVEPLLE1021IDGDIRNFEV FLSSRTPVLV ARDVKVFLPC TVNLDPKLRE IIADVRAARE QISIGGLAYP1081PLPLHEGPPR APSGYSQPPS VCSSTSFNGP FAGGVVSPQP HSSYYSGMTG PQHPFYNRPF1141FAPYLYTPRY YPGGSQHLIS RPSVKTSLPR DQNNGLEVIK EDAAEGLSSP TDSSRGSGPA1201PGPVVLLNSL NVDAVCEKLK QIEGLDQSML PQYCTTIKKA NINGRVLAQC NIDELKKEMN1261MNFGDWHLFR STVLEMRNAE SHVVPEDPRF LSESSSGPAP HGEPARRASH NELPHTELSS1321QTPYTLNFSF EELNTLGLDE GAPRHSNLSW QSQTRRTPSL SSLNSQDSSI EISKLTDKVQ1381AEYRDAYREY IAQMSQLEGG PGSTTISGRS SPHSTYYMGQ SSSGGSIHSN LEQEKGKDSE1441PKPDDGRKSF LMKRGDVIDY SSSGVSTNDA SPLDPITEED EKSDQSGSKL LPGKKSSERS1501SLFQTDLKLK GSGLRYQKLP SDEDESGTEE SDNTPLLKDD KDRKAEGKVE RVPKSPEHSA1561EPIRTFIKAK EYLSDALLDK KDSSDSGVRS SESSPNHSLH NEVADDSQLE KANLIELEDD1621SHSGKRGIPH SLSGLQDPII ARMSICSEDK KSPSECSLIA SSPEENWPAC QKAYNLNRTP1681STVTLNNNSA PANRANQNFD EMEGIRETSQ VILRPSSSPN PTTIQNENLK SMTHKRSQRS1741SYTRLSKDPP ELHAAASSES TGFGEERESI LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1771 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.