LSBio
Human KIDINS220 / ARMS Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KIDINS220 / ARMS Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KIDINS220 antibodies →
$220.00each
Catalog Number: LS-G86498-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KIDINS220 (NM_020738) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARMS | KIDINS220 | KIAA1250
Origin Species
Human
NCBI GenBank
AB033076
SwissProt
Q9ULH0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
196.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSVLISQSVI NYVEEENIPA LKALLEKCKD VDERNECGQT PLMIAAEQGN LEIVKELIKN
61GANCNLEDLD NWTALISASK EGHVHIVEEL LKCGVNLEHR DMGGWTALMW ACYKGRTDVV
121ELLLSHGANP SVTGLYSVYP IIWAAGRGHA DIVHLLLQNG AKVNCSDKYG TTPLVWAARK
181GHLECVKHLL AMGADVDQEG ANSMTALIVA VKGGYTQSVK EILKRNPNVN LTDKDGNTAL
241MIASKEGHTE IVQDLLDAGT YVNIPDRSGD TVLIGAVRGG HVEIVRALLQ KYADIDIRGQ
301DNKTALYWAV EKGNATMVRD ILQCNPDTEI CTKDGETPLI KATKMRNIEV VELLLDKGAK
361VSAVDKKGDT PLHIAIRGRS RKLAELLLRN PKDGRLLYRP NKAGETPYNI DCSHQKSILT
421QIFGARHLSP TETDGDMLGY DLYSSALADI LSEPTMQPPI CVGLYAQWGS GKSFLLKKLE
481DEMKTFAGQQ IEPLFQFSWL IVFLTLLLCG GLGLLFAFTV HPNLGIAVSL SFLALLYIFF
541IVIYFGGRRE GESWNWAWVL STRLARHIGY LELLLKLMFV NPPELPEQTT KALPVRFLFT
601DYNRLSSVGG ETSLAEMIAT LSDACEREFG FLATRLFRVF KTEDTQGKKK WKKTCCLPSF
661VIFLFIIGCI ISGITLLAIF RVDPKHLTVN AVLISIASVV GLAFVLNCRT WWQVLDSLLN
721SQRKRLHNAA SKLHKLKSEG FMKVLKCEVE LMARMAKTID SFTQNQTRLV VIIDGLDACE
781QDKVLQMLDT VRVLFSKGPF IAIFASDPHI IIKAINQNLN SVLRDSNING HDYMRNIVHL
841PVFLNSRGLS NARKFLVTSA TNGDVPCSDT TGIQEDADRR VSQNSLGEMT KLGSKTALNR
901RDTYRRRQMQ RTITRQMSFD LTKLLVTEDW FSDISPQTMR RLLNIVSVTG RLLRANQISF
961NWDRLASWIN LTEQWPYRTS WLILYLEETE GIPDQMTLKT IYERISKNIP TTKDVEPLLE
1021IDGDIRNFEV FLSSRTPVLV ARDVKVFLPC TVNLDPKLRE IIADVRAARE QISIGGLAYP
1081PLPLHEGPPR APSGYSQPPS VCSSTSFNGP FAGGVVSPQP HSSYYSGMTG PQHPFYNRPF
1141FAPYLYTPRY YPGGSQHLIS RPSVKTSLPR DQNNGLEVIK EDAAEGLSSP TDSSRGSGPA
1201PGPVVLLNSL NVDAVCEKLK QIEGLDQSML PQYCTTIKKA NINGRVLAQC NIDELKKEMN
1261MNFGDWHLFR STVLEMRNAE SHVVPEDPRF LSESSSGPAP HGEPARRASH NELPHTELSS
1321QTPYTLNFSF EELNTLGLDE GAPRHSNLSW QSQTRRTPSL SSLNSQDSSI EISKLTDKVQ
1381AEYRDAYREY IAQMSQLEGG PGSTTISGRS SPHSTYYMGQ SSSGGSIHSN LEQEKGKDSE
1441PKPDDGRKSF LMKRGDVIDY SSSGVSTNDA SPLDPITEED EKSDQSGSKL LPGKKSSERS
1501SLFQTDLKLK GSGLRYQKLP SDEDESGTEE SDNTPLLKDD KDRKAEGKVE RVPKSPEHSA
1561EPIRTFIKAK EYLSDALLDK KDSSDSGVRS SESSPNHSLH NEVADDSQLE KANLIELEDD
1621SHSGKRGIPH SLSGLQDPII ARMSICSEDK KSPSECSLIA SSPEENWPAC QKAYNLNRTP
1681STVTLNNNSA PANRANQNFD EMEGIRETSQ VILRPSSSPN PTTIQNENLK SMTHKRSQRS
1741SYTRLSKDPP ELHAAASSES TGFGEERESI L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1771 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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