Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIAA0476 / DENND4B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113651-100
Catalog Number: LS-G113651
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DENND4B (NM_014856), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DENND4B | DENN/MADD domain containing 4B | KIAA0476
- Origin Species
- Human
- NCBI GenBank
- AB007945
- SwissProt
- O75064
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 163.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEERPPRLV DYFVVAGLAG NGAPIPEETW VPEPSGPLRP PRPAEPITDV AVIARALGEE61VPQGYTCIQA SAGGHPLELS AGLLGGTQPV ICYRRGRDKP PLVELGVLYE GKERPKPGFQ121VLDTTPYSHS ANLAPPGPGH PRTYLTYRRA AEGAGLHALG ITDLCLVLPS KGEGTPHTYC181RLPRNLNPGM WGPAVYLCYK VGLAKANTLV YEAELLGRYP EEDNEAFPLP ESVPVFCLPM241GATIECWPAQ TKYPVPVFST FVLTGAAGDK VYGAALQFYE AFPRARLSER QARALGLLSA301VERGRALGGR AVRSRRAIAV LSRWPAFPAF RAFLTFLYRY SVSGPHRLPL EAHISHFIHN361VPFPSPQRPR ILVQMSPYDN LLLCQPVSSP LPLSGASFLQ LLQSLGPELA ITLLLAVLTE421HKLLVHSLRP DLLTSVCEAL VSMIFPLHWQ CPYIPLCPLV LADVLSAPVP FIVGIHSSYF481DLHDPPADVI CVDLDTNTLF QTEEKKLLSP RTLPRRPYKV LLATLTNLYQ QLDQTYTGPE541EEASLEFLLT DYEAVCGRRA RLEREVQGAF LRFMACLLKG YRVFLRPLTQ APSEGARDVD601NLFFLQGFLK SRERSSHKLY SQLLHTQMFS QFIEECSFGS ARHAALEFFD SCVEKVHPEQ661EKPEPTPLVE LEELSGSELT VFITPPEEPA LPEGSESTPQ YCYDGFPELR AELFESLQEQ721PGALPVPGPS RSAPSSPAPR RTKQEMKVAQ RMAQKSAAVP ELWARCLLGH CYGLWFLCLP781AYVRSAPSRV QALHTAYHVL RQMESGKVVL PDEVCYRVLM QLCSHYGQPV LSVRVMLEMR841QAGIVPNTIT YGYYNKAVLE SKWPSGTPGG RLRWAKLRNV VLGAAQFRQP LRERQQQQQQ901QQQQQQQQQQ EQVSAHQEAG SSQAEPYLER PSPTRPLQRQ TTWAGRSLRD PASPPGRLVK961SGSLGSARGA QPTVEAGVAH MIEALGVLEP RGSPVPWHDG SLSDLSLTGE EPLPGGSPGG1021SGSALSAQST EALEGLSGRG PKAGGRQDEA GTPRRGLGAR LQQLLTPSRH SPASRIPPPE1081LPPDLPPPAR RSPMDSLLHP RERPGSTASE SSASLGSEWD LSESSLSNLS LRRSSERLSD1141TPGSFQSPSL EILLSSCSLC RACDSLVYDE EIMAGWAPDD SNLNTTCPFC ACPFVPLLSV1201QTLDSRPSVP SPKSAGASGS KDAPVPGGPG PVLSDRRLCL ALDEPQLCNG HMGGASRRVE1261SGAWAYLSPL VLRKELESLV ENEGSEVLAL PELPSAHPII FWNLLWYFQR LRLPSILPGL1321VLASCDGPSH SQAPSPWLTP DPASVQVRLL WDVLTPDPNS CPPLYVLWRV HSQIPQRVVW1381PGPVPASLSL ALLESVLRHV GLNEVHKAVG LLLETLGPPP TGLHLQRGIY REILFLTMAA1441LGKDHVDIVA FDKKYKSAFN KLASSMGKEE LRHRRAQMPT PKAIDCRKCF GAPPECTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1496 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.