Human KIAA0476 / DENND4B Protein (Recombinant Myc-DDK (Flag))
Catalog Number: LS-G70076-20
20 µg / $1130.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human KIAA0476 / DENND4B Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G86213-20
Catalog Number: LS-G86213
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DENND4B (NM_014856) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DENND4B | DENN/MADD domain containing 4B | KIAA0476
- Origin Species
- Human
- NCBI GenBank
- AB007945
- SwissProt
- O75064
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 163.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEERPPRLV DYFVVAGLAG NGAPIPEETW VPEPSGPLRP PRPAEPITDV AVIARALGEE61VPQGYTCIQA SAGGHPLELS AGLLGGTQPV ICYRRGRDKP PLVELGVLYE GKERPKPGFQ121VLDTTPYSHS ANLAPPGPGH PRTYLTYRRA AEGAGLHALG ITDLCLVLPS KGEGTPHTYC181RLPRNLNPGM WGPAVYLCYK VGLAKANTLV YEAELLGRYP EEDNEAFPLP ESVPVFCLPM241GATIECWPAQ TKYPVPVFST FVLTGAAGDK VYGAALQFYE AFPRARLSER QARALGLLSA301VERGRALGGR AVRSRRAIAV LSRWPAFPAF RAFLTFLYRY SVSGPHRLPL EAHISHFIHN361VPFPSPQRPR ILVQMSPYDN LLLCQPVSSP LPLSGASFLQ LLQSLGPELA ITLLLAVLTE421HKLLVHSLRP DLLTSVCEAL VSMIFPLHWQ CPYIPLCPLV LADVLSAPVP FIVGIHSSYF481DLHDPPADVI CVDLDTNTLF QTEEKKLLSP RTLPRRPYKV LLATLTNLYQ QLDQTYTGPE541EEASLEFLLT DYEAVCGRRA RLEREVQGAF LRFMACLLKG YRVFLRPLTQ APSEGARDVD601NLFFLQGFLK SRERSSHKLY SQLLHTQMFS QFIEECSFGS ARHAALEFFD SCVEKVHPEQ661EKPEPTPLVE LEELSGSELT VFITPPEEPA LPEGSESTPQ YCYDGFPELR AELFESLQEQ721PGALPVPGPS RSAPSSPAPR RTKQEMKVAQ RMAQKSAAVP ELWARCLLGH CYGLWFLCLP781AYVRSAPSRV QALHTAYHVL RQMESGKVVL PDEVCYRVLM QLCSHYGQPV LSVRVMLEMR841QAGIVPNTIT YGYYNKAVLE SKWPSGTPGG RLRWAKLRNV VLGAAQFRQP LRERQQQQQQ901QQQQQQQQQQ EQVSAHQEAG SSQAEPYLER PSPTRPLQRQ TTWAGRSLRD PASPPGRLVK961SGSLGSARGA QPTVEAGVAH MIEALGVLEP RGSPVPWHDG SLSDLSLTGE EPLPGGSPGG1021SGSALSAQST EALEGLSGRG PKAGGRQDEA GTPRRGLGAR LQQLLTPSRH SPASRIPPPE1081LPPDLPPPAR RSPMDSLLHP RERPGSTASE SSASLGSEWD LSESSLSNLS LRRSSERLSD1141TPGSFQSPSL EILLSSCSLC RACDSLVYDE EIMAGWAPDD SNLNTTCPFC ACPFVPLLSV1201QTLDSRPSVP SPKSAGASGS KDAPVPGGPG PVLSDRRLCL ALDEPQLCNG HMGGASRRVE1261SGAWAYLSPL VLRKELESLV ENEGSEVLAL PELPSAHPII FWNLLWYFQR LRLPSILPGL1321VLASCDGPSH SQAPSPWLTP DPASVQVRLL WDVLTPDPNS CPPLYVLWRV HSQIPQRVVW1381PGPVPASLSL ALLESVLRHV GLNEVHKAVG LLLETLGPPP TGLHLQRGIY REILFLTMAA1441LGKDHVDIVA FDKKYKSAFN KLASSMGKEE LRHRRAQMPT PKAIDCRKCF GAPPECTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1496 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
