Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNT1 / KCa4.1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110977-100
Catalog Number: LS-G110977
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNT1 (NM_020822), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA100C15.2 | EIEE14 | ENFL5 | KCa4.1 | KCNT1 | KIAA1422 | SLACK | Slack potassium channel
- Origin Species
- Human
- NCBI GenBank
- AB037843
- SwissProt
- Q5JUK3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 139.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPLPDGARTP GGVCREARGG GYTNRTFEFD DGQCAPRRPC AGDGALLDTA GFKMSDLDSE61VLPLPPRYRF RDLLLGDPSF QNDDRVQVEF YVNENTFKER LKLFFIKNQR SSLRIRLFNF121SLKLLTCLLY IVRVLLDDPA LGIGCWGCPK QNYSFNDSSS EINWAPILWV ERKMTLWAIQ181VIVAIISFLE TMLLIYLSYK GNIWEQIFRV SFVLEMINTL PFIITIFWPP LRNLFIPVFL241NCWLAKHALE NMINDFHRAI LRTQSAMFNQ VLILFCTLLC LVFTGTCGIQ HLERAGENLS301LLTSFYFCIV TFSTVGYGDV TPKIWPSQLL VVIMICVALV VLPLQFEELV YLWMERQKSG361GNYSRHRAQT EKHVVLCVSS LKIDLLMDFL NEFYAHPRLQ DYYVVILCPT EMDVQVRRVL421QIPLWSQRVI YLQGSALKDQ DLMRAKMDNG EACFILSSRN EVDRTAADHQ TILRAWAVKD481FAPNCPLYVQ ILKPENKFHV KFADHVVCEE ECKYAMLALN CICPATSTLI TLLVHTSRGQ541EGQESPEQWQ RMYGRCSGNE VYHIRMGDSK FFREYEGKSF TYAAFHAHKK YGVCLIGLKR601EDNKSILLNP GPRHILAASD TCFYINITKE ENSAFIFKQE EKRKKRAFSG QGLHEGPARL661PVHSIIASMG TVAMDLQGTE HRPTQSGGGG GGSKLALPTE NGSGSRRPSI APVLELADSS721ALLPCDLLSD QSEDEVTPSD DEGLSVVEYV KGYPPNSPYI GSSPTLCHLL PVKAPFCCLR781LDKGCKHNSY EDAKAYGFKN KLIIVSAETA GNGLYNFIVP LRAYYRSRKE LNPIVLLLDN841KPDHHFLEAI CCFPMVYYME GSVDNLDSLL QCGIIYADNL VVVDKESTMS AEEDYMADAK901TIVNVQTMFR LFPSLSITTE LTHPSNMRFM QFRAKDSYSL ALSKLEKRER ENGSNLAFMF961RLPFAAGRVF SISMLDTLLY QSFVKDYMIT ITRLLLGLDT TPGSGYLCAM KITEGDLWIR1021TYGRLFQKLC SSSAEIPIGI YRTESHVFST SEPHDLRAQS QISVNVEDCE DTREVKGPWG1081SRAGTGGSSQ GRHTGGGDPA EHPLLRRKSL QWARRLSRKA PKQAGRAAAA EWISQQRLSL1141YRRSERQELS ELVKNRMKHL GLPTTGYEDV ANLTASDVMN RVNLGYLQDE MNDHQNTLSY1201VLINPPPDTR LEPSDIVYLI RSDPLAHVAS SSQSRKSSCS HKLSSCNPET RDETQLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1256 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.