LSBio
Human KCNT1 / KCa4.1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human KCNT1 / KCa4.1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all KCNT1 antibodies →
$220.00each
Catalog Number: LS-G92326-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KCNT1 (NM_020822) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA100C15.2 | EIEE14 | ENFL5 | KCa4.1 | KCNT1 | KIAA1422 | SLACK | Slack potassium channel
Origin Species
Human
NCBI GenBank
AB037843
SwissProt
Q5JUK3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
139.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPLPDGARTP GGVCREARGG GYTNRTFEFD DGQCAPRRPC AGDGALLDTA GFKMSDLDSE
61VLPLPPRYRF RDLLLGDPSF QNDDRVQVEF YVNENTFKER LKLFFIKNQR SSLRIRLFNF
121SLKLLTCLLY IVRVLLDDPA LGIGCWGCPK QNYSFNDSSS EINWAPILWV ERKMTLWAIQ
181VIVAIISFLE TMLLIYLSYK GNIWEQIFRV SFVLEMINTL PFIITIFWPP LRNLFIPVFL
241NCWLAKHALE NMINDFHRAI LRTQSAMFNQ VLILFCTLLC LVFTGTCGIQ HLERAGENLS
301LLTSFYFCIV TFSTVGYGDV TPKIWPSQLL VVIMICVALV VLPLQFEELV YLWMERQKSG
361GNYSRHRAQT EKHVVLCVSS LKIDLLMDFL NEFYAHPRLQ DYYVVILCPT EMDVQVRRVL
421QIPLWSQRVI YLQGSALKDQ DLMRAKMDNG EACFILSSRN EVDRTAADHQ TILRAWAVKD
481FAPNCPLYVQ ILKPENKFHV KFADHVVCEE ECKYAMLALN CICPATSTLI TLLVHTSRGQ
541EGQESPEQWQ RMYGRCSGNE VYHIRMGDSK FFREYEGKSF TYAAFHAHKK YGVCLIGLKR
601EDNKSILLNP GPRHILAASD TCFYINITKE ENSAFIFKQE EKRKKRAFSG QGLHEGPARL
661PVHSIIASMG TVAMDLQGTE HRPTQSGGGG GGSKLALPTE NGSGSRRPSI APVLELADSS
721ALLPCDLLSD QSEDEVTPSD DEGLSVVEYV KGYPPNSPYI GSSPTLCHLL PVKAPFCCLR
781LDKGCKHNSY EDAKAYGFKN KLIIVSAETA GNGLYNFIVP LRAYYRSRKE LNPIVLLLDN
841KPDHHFLEAI CCFPMVYYME GSVDNLDSLL QCGIIYADNL VVVDKESTMS AEEDYMADAK
901TIVNVQTMFR LFPSLSITTE LTHPSNMRFM QFRAKDSYSL ALSKLEKRER ENGSNLAFMF
961RLPFAAGRVF SISMLDTLLY QSFVKDYMIT ITRLLLGLDT TPGSGYLCAM KITEGDLWIR
1021TYGRLFQKLC SSSAEIPIGI YRTESHVFST SEPHDLRAQS QISVNVEDCE DTREVKGPWG
1081SRAGTGGSSQ GRHTGGGDPA EHPLLRRKSL QWARRLSRKA PKQAGRAAAA EWISQQRLSL
1141YRRSERQELS ELVKNRMKHL GLPTTGYEDV ANLTASDVMN RVNLGYLQDE MNDHQNTLSY
1201VLINPPPDTR LEPSDIVYLI RSDPLAHVAS SSQSRKSSCS HKLSSCNPET RDETQL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1256 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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