Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KCNA3 / Kv1.3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118106-100
Catalog Number: LS-G118106
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KCNA3 (NM_002232), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HGK5 | HPCN3 | HUKIII | KCNA3 | KV1.3 | MK3 | HLK3 | HUK3 | Potassium channel 3 | Type n potassium channel | PCN3
- Origin Species
- Human
- NCBI GenBank
- NM_002232
- SwissProt
- P22001
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDERLSLLRS PPPPSARHRA HPPQRPASSG GAHTLVNHGY AEPAAGRELP PDMTVVPGDH61LLEPEVADGG GAPPQGGCGG GGCDRYEPLP PSLPAAGEQD CCGERVVINI SGLRFETQLK121TLCQFPETLL GDPKRRMRYF DPLRNEYFFD RNRPSFDAIL YYYQSGGRIR RPVNVPIDIF181SEEIRFYQLG EEAMEKFRED EGFLREEERP LPRRDFQRQV WLLFEYPESS GPARGIAIVS241VLVILISIVI FCLETLPEFR DEKDYPASTS QDSFEAAGNS TSGSRAGASS FSDPFFVVET301LCIIWFSFEL LVRFFACPSK ATFSRNIMNL IDIVAIIPYF ITLGTELAER QGNGQQAMSL361AILRVIRLVR VFRIFKLSRH SKGLQILGQT LKASMRELGL LIFFLFIGVI LFSSAVYFAE421ADDPTSGFSS IPDAFWWAVV TMTTVGYGDM HPVTIGGKIV GSLCAIAGVL TIALPVPVIV481SNFNYFYHRE TEGEEQSQYM HVGSCQHLSS SAEELRKARS NSTLSKSEYM VIEEGGMNHS541AFPQTPFKTG NSTATCTTNN NPNSCVNIKK IFTDVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
575 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.