RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KARS Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125002-100
Catalog Number: LS-G125002
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Lysyl tRNA synthetase(KARS) (NM_001130089), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CMTRIB | KARS1 | KARS2 | KRS | Lysine--tRNA ligase | LysRS | KARS | KIAA0070 | Lysine tRNA ligase | Lysyl-tRNA synthetase
- Origin Species
- Human
- NCBI GenBank
- NM_005548
- SwissProt
- Q15046
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLTQAAVRLV RGSLRKTSWA EWGHRELRLG QLAPFTAPHK DKSFSDQRSE LKRRLKAEKK61VAEKEAKQKE LSEKQLSQAT AAATNHTTDN GVGPEEESVD PNQYYKIRSQ AIHQLKVNGE121DPYPHKFHVD ISLTDFIQKY SHLQPGDHLT DITLKVAGRI HAKRASGGKL IFYDLRGEGV181KLQVMANSRN YKSEEEFIHI NNKLRRGDII GVQGNPGKTK KGELSIIPYE ITLLSPCLHM241LPHLHFGLKD KETRYRQRYL DLILNDFVRQ KFIIRSKIIT YIRSFLDELG FLEIETPMMN301IIPGGAVAKP FITYHNELDM NLYMRIAPEL YHKMLVVGGI DRVYEIGRQF RNEGIDLTHN361PEFTTCEFYM AYADYHDLME ITEKMVSGMV KHITGSYKVT YHPDGPEGQA YDVDFTPPFR421RINMVEELEK ALGMKLPETN LFETEETRKI LDDICVAKAV ECPPPRTTAR LLDKLVGEFL481EVTCINPTFI CDHPQIMSPL AKWHRSKEGL TERFELFVMK KEICNAYTEL NDPMRQRQLF541EEQAKAKAAG DDEAMFIDEN FCTALEYGLP PTAGWGMGID RVAMFLTDSN NIKEVLLFPA601MKPEDKKENV ATTDTLESTT VGTSVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
625 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
