Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
JADE2 / PHF15 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113343-100
Catalog Number: LS-G113343
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHF15(JADE2) (NM_015288), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PHD finger protein 15 | PHF15 | JADE2 | Protein Jade-2 | KIAA0239
- Origin Species
- Human
- NCBI GenBank
- NM_015288
- SwissProt
- Q9NQC1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEKRRKYSI SSDNSDTTDS HATSTSASRC SKLPSSTKSG WPRQNEKKPS EVFRTDLITA61MKIPDSYQLS PDDYYILADP WRQEWEKGVQ VPAGAEAIPE PVVRILPPLE GPPAQASPSS121TMLGEGSQPD WPGGSRYDLD EIDAYWLELI NSELKEMERP ELDELTLERV LEELETLCHQ181NMARAIETQE GLGIEYDEDV VCDVCRSPEG EDGNEMVFCD KCNVCVHQAC YGILKVPTGS241WLCRTCALGV QPKCLLCPKR GGALKPTRSG TKWVHVSCAL WIPEVSIGCP EKMEPITKIS301HIPASRWALS CSLCKECTGT CIQCSMPSCV TAFHVTCAFD HGLEMRTILA DNDEVKFKSF361CQEHSDGGPR NEPTSEPTEP SQAGEDLEKV TLRKQRLQQL EEDFYELVEP AEVAERLDLA421EALVDFIYQY WKLKRKANAN QPLLTPKTDE VDNLAQQEQD VLYRRLKLFT HLRQDLERVR481NLCYMVTRRE RTKHAICKLQ EQIFHLQMKL IEQDLCRAGL STSFPIDGTF FNSWLAQSVQ541ITAENMAMSE WPLNNGHRED PAPGLLSEEL LQDEETLLSF MRDPSLRPGD PARKARGRTR601LPAKKKPPPP PPQDGPGSRT TPDKAPKKTW GQDAGSGKGG QGPPTRKPPR RTSSHLPSSP661AAGDCPILAT PESPPPLAPE TPDEAASVAA DSDVQVPGPA ASPKPLGRLR PPRESKVTRR721LPGARPDAGM GPPSAVAERP KVSLHFDTET DGYFSDGEMS DSDVEAEDGG VQRGPREAGA781EEVVRMGVLA STRTRPLEQKLISEEDLAANDILDYKDDDDKV
791 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.