LSBio
Human JADE2 / PHF15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human JADE2 / PHF15 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all JADE2 antibodies →
$153.00each
Catalog Number: LS-G77507-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PHF15(JADE2) (NM_015288) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PHD finger protein 15 | PHF15 | JADE2 | Protein Jade-2 | KIAA0239
Origin Species
Human
NCBI GenBank
NM_015288
SwissProt
Q9NQC1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEKRRKYSI SSDNSDTTDS HATSTSASRC SKLPSSTKSG WPRQNEKKPS EVFRTDLITA
61MKIPDSYQLS PDDYYILADP WRQEWEKGVQ VPAGAEAIPE PVVRILPPLE GPPAQASPSS
121TMLGEGSQPD WPGGSRYDLD EIDAYWLELI NSELKEMERP ELDELTLERV LEELETLCHQ
181NMARAIETQE GLGIEYDEDV VCDVCRSPEG EDGNEMVFCD KCNVCVHQAC YGILKVPTGS
241WLCRTCALGV QPKCLLCPKR GGALKPTRSG TKWVHVSCAL WIPEVSIGCP EKMEPITKIS
301HIPASRWALS CSLCKECTGT CIQCSMPSCV TAFHVTCAFD HGLEMRTILA DNDEVKFKSF
361CQEHSDGGPR NEPTSEPTEP SQAGEDLEKV TLRKQRLQQL EEDFYELVEP AEVAERLDLA
421EALVDFIYQY WKLKRKANAN QPLLTPKTDE VDNLAQQEQD VLYRRLKLFT HLRQDLERVR
481NLCYMVTRRE RTKHAICKLQ EQIFHLQMKL IEQDLCRAGL STSFPIDGTF FNSWLAQSVQ
541ITAENMAMSE WPLNNGHRED PAPGLLSEEL LQDEETLLSF MRDPSLRPGD PARKARGRTR
601LPAKKKPPPP PPQDGPGSRT TPDKAPKKTW GQDAGSGKGG QGPPTRKPPR RTSSHLPSSP
661AAGDCPILAT PESPPPLAPE TPDEAASVAA DSDVQVPGPA ASPKPLGRLR PPRESKVTRR
721LPGARPDAGM GPPSAVAERP KVSLHFDTET DGYFSDGEMS DSDVEAEDGG VQRGPREAGA
781EEVVRMGVLA S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

791 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.