Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ITGAD / CD11d Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116023-100
Catalog Number: LS-G116023
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CD11d(ITGAD) (NM_005353), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ADB2 | CD11D | Integrin | alpha D | Leukointegrin alpha D | Leukointegrin alpha d chain | CD11d antigen | Integrin alpha-D | ITGAD
- Origin Species
- Human
- NCBI GenBank
- U37028
- SwissProt
- Q13349
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 126.76 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTFGTVLLLS VLASYHGFNL DVEEPTIFQE DAGGFGQSVV QFGGSRLVVG APLEVVAANQ61TGRLYDCAAA TGMCQPIPLH IRPEAVNMSL GLTLAASTNG SRLLACGPTL HRVCGENSYS121KGSCLLLGSR WEIIQTVPDA TPECPHQEMD IVFLIDGSGS IDQNDFNQMK GFVQAVMGQF181EGTDTLFALM QYSNLLKIHF TFTQFRTSPS QQSLVDPIVQ LKGLTFTATG ILTVVTQLFH241HKNGARKSAK KILIVITDGQ KYKDPLEYSD VIPQAEKAGI IRYAIGVGHA FQGPTARQEL301NTISSAPPQD HVFKVDNFAA LGSIQKQLQE KIYAVEGTQS RASSSFQHEM SQEGFSTALT361MDGLFLGAVG SFSWSGGAFL YPPNMSPTFI NMSQENVDMR DSYLGYSTEL ALWKGVQNLV421LGAPRYQHTG KAVIFTQVSR QWRKKAEVTG TQIGSYFGAS LCSVDVDSDG STDLILIGAP481HYYEQTRGGQ VSVCPLPRGR VQWQCDAVLR GEQGHPWGRF GAALTVLGDV NEDKLIDVAI541GAPGEQENRG AVYLFHGASE SGISPSHSQR IASSQLSPRL QYFGQALSGG QDLTQDGLMD601LAVGARGQVL LLRSLPVLKV GVAMRFSPVE VAKAVYRCWE EKPSALEAGD ATVCLTIQKS661SLDQLGDIQS SVRFDLALDP GRLTSRAIFN ETKNPTLTRR KTLGLGIHCE TLKLLLPDCV721EDVVSPIILH LNFSLVREPI PSPQNLRPVL AVGSQDLFTA SLPFEKNCGQ DGLCEGDLGV781TLSFSGLQTL TVGSSLELNV IVTVWNAGED SYGTVVSLYY PAGLSHRRVS GAQKQPHQSA841LRLACETVPT EDEGLRSSRC SVNHPIFHEG SNGTFIVTFD VSYKATLGDR MLMRASASSE901NNKASSSKAT FQLELPVKYA VYTMISRQEE STKYFNFATS DEKKMKEAEH RYRVNNLSQR961DLAISINFWV PVLLNGVAVW DVVMEAPSQS LPCVSERKPP QHSDFLTQIS RSPMLDCSIA1021DCLQFRCDVP SFSVQEELDF TLKGNLSFGW VRETLQKKVL VVSVAEITFD TSVYSQLPGQ1081EAFMRAQMEM VLEEDEVYNA IPIIMGSSVG ALLLLALITA TLYKLGFFKR HYKEMLEDKP1141EDTATFSGDD FSCVAPNVPL STRTRPLEQKLISEEDLAANDILDYKDDDDKV
1161 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.