LSBio
Human ITGAD / CD11d Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ITGAD / CD11d Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ITGAD antibodies →
$220.00each
Catalog Number: LS-G81545-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CD11d(ITGAD) (NM_005353) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADB2 | CD11D | Integrin | alpha D | Leukointegrin alpha D | Leukointegrin alpha d chain | CD11d antigen | Integrin alpha-D | ITGAD
Origin Species
Human
NCBI GenBank
U37028
SwissProt
Q13349

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
126.76 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTFGTVLLLS VLASYHGFNL DVEEPTIFQE DAGGFGQSVV QFGGSRLVVG APLEVVAANQ
61TGRLYDCAAA TGMCQPIPLH IRPEAVNMSL GLTLAASTNG SRLLACGPTL HRVCGENSYS
121KGSCLLLGSR WEIIQTVPDA TPECPHQEMD IVFLIDGSGS IDQNDFNQMK GFVQAVMGQF
181EGTDTLFALM QYSNLLKIHF TFTQFRTSPS QQSLVDPIVQ LKGLTFTATG ILTVVTQLFH
241HKNGARKSAK KILIVITDGQ KYKDPLEYSD VIPQAEKAGI IRYAIGVGHA FQGPTARQEL
301NTISSAPPQD HVFKVDNFAA LGSIQKQLQE KIYAVEGTQS RASSSFQHEM SQEGFSTALT
361MDGLFLGAVG SFSWSGGAFL YPPNMSPTFI NMSQENVDMR DSYLGYSTEL ALWKGVQNLV
421LGAPRYQHTG KAVIFTQVSR QWRKKAEVTG TQIGSYFGAS LCSVDVDSDG STDLILIGAP
481HYYEQTRGGQ VSVCPLPRGR VQWQCDAVLR GEQGHPWGRF GAALTVLGDV NEDKLIDVAI
541GAPGEQENRG AVYLFHGASE SGISPSHSQR IASSQLSPRL QYFGQALSGG QDLTQDGLMD
601LAVGARGQVL LLRSLPVLKV GVAMRFSPVE VAKAVYRCWE EKPSALEAGD ATVCLTIQKS
661SLDQLGDIQS SVRFDLALDP GRLTSRAIFN ETKNPTLTRR KTLGLGIHCE TLKLLLPDCV
721EDVVSPIILH LNFSLVREPI PSPQNLRPVL AVGSQDLFTA SLPFEKNCGQ DGLCEGDLGV
781TLSFSGLQTL TVGSSLELNV IVTVWNAGED SYGTVVSLYY PAGLSHRRVS GAQKQPHQSA
841LRLACETVPT EDEGLRSSRC SVNHPIFHEG SNGTFIVTFD VSYKATLGDR MLMRASASSE
901NNKASSSKAT FQLELPVKYA VYTMISRQEE STKYFNFATS DEKKMKEAEH RYRVNNLSQR
961DLAISINFWV PVLLNGVAVW DVVMEAPSQS LPCVSERKPP QHSDFLTQIS RSPMLDCSIA
1021DCLQFRCDVP SFSVQEELDF TLKGNLSFGW VRETLQKKVL VVSVAEITFD TSVYSQLPGQ
1081EAFMRAQMEM VLEEDEVYNA IPIIMGSSVG ALLLLALITA TLYKLGFFKR HYKEMLEDKP
1141EDTATFSGDD FSCVAPNVPL S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1161 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.