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IQCE Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IQCE Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IQCE antibodies →
$725.00each
Catalog Number: LS-G118888-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IQCE (NM_001100390), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IQCE | KIAA1023 | IQ motif containing E | IQ domain-containing protein E
Origin Species
Human
NCBI GenBank
AB028946
SwissProt
Q6IPM2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFLGTGEPAL DTKAKRKAFH KPPPTSPKSP YLSKPRKVAS WRSLRTAGSM PLGGRASLTP
61QKLWLGTAKP GSLTQALNSP LTWEHAWTGV PGGTPDCLTD TFRVKRPHLR RSASNGHVPG
121TPVYREKEDM YDEIIELKKS LHVQKSDVDL MRTKLRRLEE ENSRKDRQIE QLLDPSRGTD
181FVRTLAEKRP DASWVINGLK QRILKLEQQC KEKDGTISKL QTDMKTTNLE EMRIAMETYY
241EEVHRLQTLL ASSETTGKKP LGEKKTGAKR QKKMGSALLS LSRSVQELTE ENQSLKEDLD
301RVLSTSPTIS KTQGYVEWSK PRLLRRIVEL EKKLSVMESS KSHAAEPVRS HPPACLASSS
361ALHRQPRGDR NKDHERLRGA VRDLKEERTA LQEQLLQRDL EVKQLLQAKA DLEKELECAR
421EGEEERRERE EVLREEIQTL TSKLQELQEM KKEEKEDCPE VPHKAQELPA PTPSSRHCEQ
481DWPPDSSEEG LPRPRSPCSD GRRDAAARVL QAQWKVYKHK KKKAVLDEAA VVLQAAFRGH
541LTRTKLLASK AHGSEPPSVP GLPDQSSPVP RVPSPIAQAT GSPVQEEAIV IIQSALRAHL
601ARARHSATGK RTTTAASTRR RSASATHGDA SSPPFLAALP DPSPSGPQAL APLPGDDVNS
661DDSDDIVIAP SLPTKNFPV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

679 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.