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IQCE Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IQCE Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IQCE antibodies →
$504.00each
Catalog Number: LS-G106194-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IQCE (NM_152558), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IQCE | KIAA1023 | IQ motif containing E | IQ domain-containing protein E
Origin Species
Human
NCBI GenBank
AB028946
SwissProt
Q6IPM2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFLGTGEPAL DTGDDSLSAV TFDSDVETKA KRKAFHKPPP TSPKSPYLSK PRKVASWRSL
61RTAGSMPLGG RASLTPQKLW LGTAKPGSLT QALNSPLTWE HAWTGVPGGT PDCLTDTFRV
121KRPHLRRSAS NGHVPGTPVY REKEDMYDEI IELKKSLHVQ KSDVDLMRTK LRRLEEENSR
181KDRQIEQLLD PSRGTDFVRT LAEKRPDASW VINGLKQRIL KLEQQCKEKD GTIRKLQTDM
241KTTNLEEMRI AMETYYEEVH RLQTLLASSE TTGKKPLGEK KTGAKRQKKM GSALLSLSRS
301VQELTEENQS LKEDLDRVLS TSPTISKTQG YVEWSKPRLL RRIVELEKKL SVMESSKSHA
361AEPVRSHPPA CLASSSALHR QPRGDRNKDH ERLRGAVRDL KEERTALQEQ LLQRDLEVKQ
421LLQAKADLEK ELECAREGEE ERREREEVLR EEIQTLTSKL QELQEMKKEE KEDCPEVPHK
481AQELPAPTPS SRHCEQDWPP DSSEEGLPRP RSPCSDGRRD AAARVLQAQW KVYKHKKKKA
541VLDEAAVVLQ AAFRGHLTRT KLLASKAHGS EPPSVPGLSD QSSPVPRVPS PIAQATGSPV
601QEEAIVIIQS ALRAHLARAR HSATGKRTTT AASTRRRSAS ATHGDASSPP FLAALPDPSP
661SGPQALAPLP GDDVNSDDSD DIVIAPSLPT KNFPV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

695 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.