LSBio
IQCC Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IQCC Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IQCC antibodies →
$504.00each
Catalog Number: LS-G128216-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IQCC (NM_001160042), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IQ domain-containing protein C | IQCC | IQ motif containing C | RP4-622L5.6
Origin Species
Human
NCBI GenBank
NM_018134

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPELLVRKV SALQVRGRAR GSQDFSFREI MGSRTWTSQR DATTFSPLTA RQPLEIPAVP
61NRAEESWDPR TPCPARPHSH HTLAPDQGNG KSLKACVRGF LVRRQFQSLR AEYEAIVREV
121EGDLGTLQWT EGRIPRPRFL PEKAKSHQTW KAGDRVANPE QGLWNHFPCE ESEGEATWEE
181MVLKKSGESS ANQGSLCRDH SSWLQMKQNR KPSQEKTRDT TRMENPEATD QRLPHSQPQL
241QELQYHRSHL AMELLWLQQA INSRKEYLLL KQTLRSPEAG PIREEPRVFL EHGEQACERD
301QSQPSAPLED QSYRDRTTGE LEQEDDSCHR VKSPHRSPGS LATTQKNIAG AKCREPCYSK
361SGPPSSIPSN SQALGDRLTK GPDDGRQTFG GTCLLQMKIL EDQTPRGLKP RNHCPRKSRT
421QLSALYEDSN IKEMSPRKLD HKEPDCRTVR TQELGLSEDH IIWDGTLGGP EHSVLDLWRT
481KPPKGQAPTD RSSRDGTSNE PSHEGQKKQR TIPWRSKSPE ILSSTKAGCT GEEQWRGRPW
541KTEPPG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

546 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.