LSBio
IQCC Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IQCC Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IQCC antibodies →
$725.00each
Catalog Number: LS-G111963-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IQCC (NM_018134), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IQ domain-containing protein C | IQCC | IQ motif containing C | RP4-622L5.6
Origin Species
Human
NCBI GenBank
NM_018134

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPELLVRKV SALQACVRGF LVRRQFQSLR AEYEAIVREV EGDLGTLQWT EGRIPRPRFL
61PEKAKSHQTW KAGDRVANPE QGLWNHFPCE ESEGEATWEE MVLKKSGESS ANQGSLCRDH
121SSWLQMKQNR KPSQEKTRDT TRMENPEATD QRLPHSQPQL QELQYHRSHL AMELLWLQQA
181INSRKEYLLL KQTLRSPEAG PIREEPRVFL EHGEQACERD QSQPSAPLED QSYRDRTTGE
241LEQEDDSCHR VKSPHRSPGS LATTQKNIAG AKCREPCYSK SGPPSSIPSN SQALGDRLTK
301GPDDGRQTFG GTCLLQMKIL EDQTPRGLKP RNHCPRKSRT QLSALYEDSN IKEMSPRKLD
361HKEPDCRTVR TQELGLSEDH IIWDGTLGGP EHSVLDLWRT KPPKGQAPTD RSSRDGTSNE
421PSHEGQKKQR TIPWRSKSPE ILSSTKAGCT GEEQWRGRPW KTELPG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

466 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.