Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IPO5 / RANBP5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118078-100
Catalog Number: LS-G118078
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Karyopherin beta 3(IPO5) (NM_002271), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IMB3 | Importin 5 | Importin subunit beta-3 | Imp5 | Importin-5 | IPO5 | Importin beta-3 subunit | Karyopherin beta-3 | KPNB3 | Karyopherin (importin) beta 3 | Pse1 | RAN binding protein 5 | Ran-binding protein 5 | Ran_GTP binding protein 5 | RANBP5
- Origin Species
- Human
- NCBI GenBank
- NM_002271
- SwissProt
- O00410
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 125.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPEDQVGKLE ATENTISAMA AAAAEQQQFY LLLGNLLSPD NVVRKQAEET YENIPGQSKI61TFLLQAIRNT TAAEEARQMA AVLLRRLLSS AFDEVYPALP SDVQTAIKSE LLMIIQMETQ121SSMRKKVCDI AAELARNLID EDGNNQWPEG LKFLFDSVSS QNVGLREAAL HIFWNFPGIF181GNQQQHYLDV IKRMLVQCMQ DQEHPSIRTL SARATAAFIL ANEHNVALFK HFADLLPGFL241QAVNDSCYQN DDSVLKSLVE IADTVPKYLR PHLEATLQLS LKLCGDTSLN NMQRQLALEV301IVTLSETAAA MLRKHTNIVA QTIPQMLAMM VDLEEDEDWA NADELEDDDF DSNAVAGESA361LDRMACGLGG KLVLPMIKEH IMQMLQNPDW KYRHAGLMAL SAIGEGCHQQ MEGILNEIVN421FVLLFLQDPH PRVRYAACNA VGQMATDFAP GFQKKFHEKV IAALLQTMED QGNQRVQAHA481AAALINFTED CPKSLLIPYL DNLVKHLHSI MVLKLQELIQ KGTKLVLEQV VTSIASVADT541AEEKFVPYYD LFMPSRKHIV ENAVQKELRL LRGKTIECIS LIGLAVGKEK FMQDASDVMQ601LLLKTQTDFN DMEDDDPQIS YMISAWARMC KILGKEFQQY LPVVMGPLMK TASIKPEVAL661LDTQDMENMS DDDGWEFVNL GDQQSFGIKT AGLEEKSTAC QMLVCYAKEL KEGFVEYTEQ721VVKLMVPLLK FYFHDGVRVA AAESMPLLLE CARVRGPEYL TQMWHFMCDA LIKAIGTEPD781SDVLSEIMHS FAKCIEVMGD GCLNNEHFEE LGGILKAKLE EHFKNQELRQ VKRQDEDYDE841QVEESLQDED DNDVYILTKV SDILHSIFSS YKEKVLPWFE QLLPLIVNLI CPHRPWPDRQ901WGLCIFDDVI EHCSPASFKY AEYFLRPMLQ YVCDNSPEVR QAAAYGLGVM AQYGGDNYRP961FCTEALPLLV RVIQSADSKT KENVNATENC ISAVGKIMKF KPDCVNVEEV LPHWLSWLPL1021HEDKEEAVQT FNYLCDLIES NHPIVLGPNN TNLPKIFSII AEGEMHEAIK HEDPCAKRLA1081NVVRQVQTSG GLWTECIAQL SPEQQAAIQE LLNSATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1115 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.