IPO5 / RANBP5 Antibody
Catalog Number: LS-C331805
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human IPO5 / RANBP5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G76754-20
Catalog Number: LS-G76754
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Karyopherin beta 3(IPO5) (NM_002271) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IMB3 | Importin 5 | Importin subunit beta-3 | Imp5 | Importin-5 | IPO5 | Importin beta-3 subunit | Karyopherin beta-3 | KPNB3 | Karyopherin (importin) beta 3 | Pse1 | RAN binding protein 5 | Ran-binding protein 5 | Ran_GTP binding protein 5 | RANBP5
- Origin Species
- Human
- NCBI GenBank
- NM_002271
- SwissProt
- O00410
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 125.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPEDQVGKLE ATENTISAMA AAAAEQQQFY LLLGNLLSPD NVVRKQAEET YENIPGQSKI61TFLLQAIRNT TAAEEARQMA AVLLRRLLSS AFDEVYPALP SDVQTAIKSE LLMIIQMETQ121SSMRKKVCDI AAELARNLID EDGNNQWPEG LKFLFDSVSS QNVGLREAAL HIFWNFPGIF181GNQQQHYLDV IKRMLVQCMQ DQEHPSIRTL SARATAAFIL ANEHNVALFK HFADLLPGFL241QAVNDSCYQN DDSVLKSLVE IADTVPKYLR PHLEATLQLS LKLCGDTSLN NMQRQLALEV301IVTLSETAAA MLRKHTNIVA QTIPQMLAMM VDLEEDEDWA NADELEDDDF DSNAVAGESA361LDRMACGLGG KLVLPMIKEH IMQMLQNPDW KYRHAGLMAL SAIGEGCHQQ MEGILNEIVN421FVLLFLQDPH PRVRYAACNA VGQMATDFAP GFQKKFHEKV IAALLQTMED QGNQRVQAHA481AAALINFTED CPKSLLIPYL DNLVKHLHSI MVLKLQELIQ KGTKLVLEQV VTSIASVADT541AEEKFVPYYD LFMPSRKHIV ENAVQKELRL LRGKTIECIS LIGLAVGKEK FMQDASDVMQ601LLLKTQTDFN DMEDDDPQIS YMISAWARMC KILGKEFQQY LPVVMGPLMK TASIKPEVAL661LDTQDMENMS DDDGWEFVNL GDQQSFGIKT AGLEEKSTAC QMLVCYAKEL KEGFVEYTEQ721VVKLMVPLLK FYFHDGVRVA AAESMPLLLE CARVRGPEYL TQMWHFMCDA LIKAIGTEPD781SDVLSEIMHS FAKCIEVMGD GCLNNEHFEE LGGILKAKLE EHFKNQELRQ VKRQDEDYDE841QVEESLQDED DNDVYILTKV SDILHSIFSS YKEKVLPWFE QLLPLIVNLI CPHRPWPDRQ901WGLCIFDDVI EHCSPASFKY AEYFLRPMLQ YVCDNSPEVR QAAAYGLGVM AQYGGDNYRP961FCTEALPLLV RVIQSADSKT KENVNATENC ISAVGKIMKF KPDCVNVEEV LPHWLSWLPL1021HEDKEEAVQT FNYLCDLIES NHPIVLGPNN TNLPKIFSII AEGEMHEAIK HEDPCAKRLA1081NVVRQVQTSG GLWTECIAQL SPEQQAAIQE LLNSATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1115 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
