Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
INPP5D / SHIP1 / SHIP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115897-100
Catalog Number: LS-G115897
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SHIP(INPP5D) (NM_005541), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- p150Ship | INPP5D | SHIP | SHIP1 | SHIP-1 | Hp51CN | SIP-145
- Origin Species
- Human
- NCBI GenBank
- NM_005541
- SwissProt
- Q92835
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 133 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVPCWNHGNI TRSKAEELLS RTGKDGSFLV RASESISRAY ALCVLYRNCV YTYRILPNED61DKFTVQASEG VSMRFFTKLD QLIEFYKKEN MGLVTHLQYP VPLEEEDTGD DPEEDTESVV121SPPELPPRNI PLTASSCEAK EVPFSNENPR ATETSRPSLS ETLFQRLQSM DTSGLPEEHL181KAIQDYLSTQ LAQDSEFVKT GSSSLPHLKK LTTLLCKELY GEVIRTLPSL ESLQRLFDQQ241LSPGLRPRPQ VPGEANPINM VSKLSQLTSL LSSIEDKVKA LLHEGPESPH RPSLIPPVTF301EVKAESLGIP QKMQLKVDVE SGKLIIKKSK DGSEDKFYSH KKILQLIKSQ KFLNKLVILV361ETEKEKILRK EYVFADSKKR EGFCQLLQQM KNKHSEQPEP DMITIFIGTW NMGNAPPPKK421ITSWFLSKGQ GKTRDDSADY IPHDIYVIGT QEDPLSEKEW LEILKHSLQE ITSVTFKTVA481IHTLWNIRIV VLAKPEHENR ISHICTDNVK TGIANTLGNK GAVGVSFMFN GTSLGFVNSH541LTSGSEKKLR RNQNYMNILR FLALGDKKLS PFNITHRFTH LFWFGDLNYR VDLPTWEAET601IIQKIKQQQY ADLLSHDQLL TERREQKVFL HFEEEEITFA PTYRFERLTR DKYAYTKQKA661TGMKYNLPSW CDRVLWKSYP LVHVVCQSYG STSDIMTSDH SPVFATFEAG VTSQFVSKNG721PGTVDSQGQI EFLRCYATLK TKSQTKFYLE FHSSCLESFV KSQEGENEEG SEGELVVKFG781ETLPKLKPII SDPEYLLDQH ILISIKSSDS DESYGEGCIA LRLEATETQL PIYTPLTHHG841ELTGHFQGEI KLQTSQGKTR EKLYDFVKTE RDESSGPKTL KSLTSHDPMK QWEVTSRAPP901CSGSSITEII NPNYMGVGPF GPPMPLHVKQ TLSPDQQPTA WSYDQPPKDS PLGPCRGESP961PTPPGQPPIS PKKFLPSTAN RGLPPRTQES RPSDLGKNAG DTLPQEDLPL TKPEMFENPL1021YGSLSSFPKP APRKDQESPK MPRKEPPPCP EPGILSPSIV LTKAQEADRG EGPGKQVPAP1081RLRSFTCSSS AEGRAAGGDK SQGKPKTPVS SQAPVPAKRP IKPSRSEINQ QTPPTPTPRP1141PLPVKSPAVL HLQHSKGRDY RDNTELPHHG KHRPEEGPPG PLGRTAMQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1188 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.