LSBio
ING1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ING1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ING1 antibodies →
$504.00each
Catalog Number: LS-G103717-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ING1 (NM_198219), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Growth inhibitor ING1 | Growth inhibitor p33ing1 | ING1 | Inhibitor of growth protein 1 | p33 | p33ING1 | p47ING1a | Tumor suppressor ING1 | p33ING1b | Growth inhibitory protein ING1 | Inhibitor of growth 1 | p24ING1c
Origin Species
Human
NCBI GenBank
NM_005537
SwissProt
Q9UK53

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
31.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MLSPANGEQL HLVNYVEDYL DSIESLPFDL QRNVSLMREI DAKYQEILKE LDECYERFSR
61ETDGAQKRRM LHCVQRALIR SQELGDEKIQ IVSQMVELVE NRTRQVDSHV ELFEAQQELG
121DTAGNSGKAG ADRPKGEAAA QADKPNSKRS RRQRNNENRE NASSNHDHDD GASGTPKEKK
181AKTSKKKKRS KAKAEREASP ADLPIDPNEP TYCLCNQVSY GEMIGCDNDE CPIEWFHFSC
241VGLNHKPKGK WYCPKCRGEN EKTMDKALEK SKKERAYNR
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

279 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.