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IGF2BP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IGF2BP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G121952-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IGF2BP2 (NM_001007225), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IMP2 | IGF2 mRNA-binding protein 2 | p62 | VICKZ2 | IGF-II mRNA-binding protein 2 | IGF2BP2 | IMP-2 | VICKZ family member 2
Origin Species
Human
NCBI GenBank
NM_006548
SwissProt
Q9Y6M1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMNKLYIGNL SPAVTADDLR QLFGDRKLPL AGQVLLKSGY AFVDYPDQNW AIRAIETLSG
61KVELHGKIME VDYSVSKKLR SRKIQIRNIP PHLQWEVLDG LLAQYGTVEN VEQVNTDTET
121AVVNVTYATR EEAKIAMEKL SGHQFENYSF KISYIPDEEV SSPSPPQRAQ RGDHSSREQG
181HAPGGTSQAR QIDFPLRILV PTQFVGAIIG KEGLTIKNIT KQTQSRVDIH RKENSGAAEK
241PVTIHATPEG TSEACRMILE IMQKEADETK LAEEIPLKIL AHNGLVGRLI GKEGRNLKKI
301EHETGTKITI SSLQDLSIYN PERTITVKGT VEACASAEIE IMKKLREAFE NDMLAVNTHS
361GYFSSLYPHH QFGPFPHHHS YPEQEIVNLF IPTQAVGAII GKKGAHIKQL ARFAGASIKI
421APAEGPDVSE RMVIITGPPE AQFKAQGRIF GKLKEENFFN PKEEVKLEAH IRVPSSTAGR
481VIGKGGKTVN ELQNLTSAEV IVPRDQTPDE NEEVIVRIIG HFFASQTAQR KIREIVQQVK
541QQEQKYPQGV ASQRSK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

556 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.