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IGF2BP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IGF2BP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IGF2BP2 antibodies →
$504.00each
Catalog Number: LS-G100844-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IGF2BP2 (NM_006548), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IMP2 | IGF2 mRNA-binding protein 2 | p62 | VICKZ2 | IGF-II mRNA-binding protein 2 | IGF2BP2 | IMP-2 | VICKZ family member 2
Origin Species
Hu
NCBI GenBank
NM_006548
SwissProt
Q9Y6M1

Protein

Expression System
HEK293
Fusion Tag
FLAG, Myc
Molecular Weight
65.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNKLYIGNLS PAVTADDLRQ LFGDRKLPLA GQVLLKSGYA FVDYPDQNWA IRAIETLSGK
61VELHGKIMEV DYSVSKKLRS RKIQIRNIPP HLQWEVLDGL LAQYGTVENV EQVNTDTETA
121VVNVTYATRE EAKIAMEKLS GHQFENYSFK ISYIPDEEVS SPSPPQRAQR GDHSSREQGH
181APGGTSQARQ IDFPLRILVP TQFVGAIIGK EGLTIKNITK QTQSRVDIHR KENSGAAEKP
241VTIHATPEGT SEACRMILEI MQKEADETKL AEEIPLKILA HNGLVGRLIG KEGRNLKKIE
301HETGTKITIS SLQDLSIYNP ERTITVKGTV EACASAEIEI MKKLREAFEN DMLAVNQQAN
361LIPGLNLSAL GIFSTGLSVL SPPAGPRGAP PAAPYHPFTT HSGYFSSLYP HHQFGPFPHH
421HSYPEQEIVN LFIPTQAVGA IIGKKGAHIK QLARFAGASI KIAPAEGPDV SERMVIITGP
481PEAQFKAQGR IFGKLKEENF FNPKEEVKLE AHIRVPSSTA GRVIGKGGKT VNELQNLTSA
541EVIVPRDQTP DENEEVIVRI IGHFFASQTA QRKIREIVQQ VKQQEQKYPQ GVASQRSK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

598 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.