Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IDO2 / INDOL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102543-100
Catalog Number: LS-G102543
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human INDOL1(IDO2) (NM_194294), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IDO-2 | IDO2 | Indoleamine 2 | 3-dioxygenase 2 | INDOL1
- Origin Species
- Human
- SwissProt
- Q6ZQW0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLHFHYYDTS NKIMEPHRPN VKTAVPLSLE SYHISEEYGF LLPDSLKELP DHYRPWMEIA61NKLPQLIDAH QLQAHVDKMP LLSCQFLKGH REQRLAHLVL SFLTMGYVWQ EGEAQPAEVL121PRNLALPFVE VSRNLGLPPI LVHSDLVLTN WTKKDPDGFL EIGNLETIIS FPGGESLHGF181ILVTALVEKE AVPGIKALVQ ATNAILQPNQ EALLQALQRL RLSIQDITKT LGQMHDYVDP241DIFYAGIRIF LSGWKDNPAM PAGLMYEGVS QEPLKYSGGS AAQSTVLHAF DEFLGIRHSK301ESGDFLYRMR DYMPPSHKAF IEDIHSAPSL RDYILSSGQD HLLTAYNQCV QALAELRSYH361ITMVTKYLIT AAAKAKHGKP NHLPGPPQAL KDRGTGGTAV MSFLKSVRDK TLESILHPRGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
420 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.