Western blot of Mouse Anti-IDO2 antibody. Lane 1: HEK293 no transgene cell extracts. Lane 2: mouse IDO-2 transgene expressed in 293HEK. Lane 3: mouse IDO-1 transgene expressed in 293HEK. Primary antibody: IDO2 monoclonal Antibody. Secondary antibody: IRDye800 mouse secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 44.4 kDa, ~60 kDa for IDO 2. Other band(s): IDO2 splice variants and isoforms.
IDO2 / INDOL1 Antibody (clone 1HC)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C153838-100
Catalog Number: LS-C153838
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- Description
- INDOL1 antibody LS-C153838 is an unconjugated mouse monoclonal antibody to mouse INDOL1 (IDO2). Validated for ELISA, IF and WB. Cited in 1 publication.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- IDO-2 | IDO2 | Indoleamine 2,3-dioxygenase 2 | INDOL1
- Recommended Dilution: ELISA
- 1:5000 - 1:50000
- Recommended Dilution: IF/ICC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1500
- UniProt Number
- Q6ZQW0
- NCBI GenBank
- NP_919270.2
- SwissProt
- Q6ZQW0
Target
- Target
- Mouse IDO2 / INDOL1
- Antigen Species
- Mouse
- Gene Name
- IDO2
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 1HC
- Isotype
- IgG2a
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Anti-IDO2 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with complete mouse IDO2 protein followed by hybridoma development.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Zinc protoporphyrin IX stimulates tumor immunity by disrupting the immunosuppressive enzyme indoleamine 2,3-dioxygenase. Metz R, Duhadaway JB, Rust S, Munn DH, Muller AJ, Mautino M, Prendergast GC. Molecular cancer therapeutics. 2010;9:1864-71. PubMed: 20530717 PMC: PMC2885048