LSBio
Human ZIMP7 / ZMIZ2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ZIMP7 / ZMIZ2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ZMIZ2 antibodies →
$220.00each
Catalog Number: LS-G88530-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZMIZ2 (NM_031449) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NET27 | HZIMP7 | PIAS-like protein Zimp7 | TRAFIP20 | ZIMP7 | KIAA1886 | ZMIZ2
Origin Species
Human
NCBI GenBank
BC021924
SwissProt
Q8NF64

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSMNPMKPA LPPAPHGDGS FAYESVPWQQ SATQPAGSLS VVTTVWGVGN ATQSQVLGNP
61MGPAGSPSGS SMMPGVAGGS SALTSPQCLG QQAFAEGGAN KGYVQQGVYS RGGYPGAPGF
121TTGYAGGPGG LGLPSHAARP STDFTQAAAA AAVAAAAATA TATATATVAA LQEKQSQELS
181QYGAMGAGQS FNSQFLQHGG PRGPSVPAGM NPTGIGGVMG PSGLSPLAMN PTRAAGMTPL
241YAGQRLPQHG YPGPPQAQPL PRQGVKRTYS EVYPGQQYLQ GGQYAPSTAQ FAPSPGQPPA
301PSPSYPGHRL PLQQGMTQSL SVPGPTGLHY KPTEQFNGQG ASFNGGSVSY SQPGLSGPTR
361SIPGYPSSPL PGNPTPPMTP SSSVPYMSPN QEVKSPFLPD LKPNLNSLHS SPSGSGPCDE
421LRLTFPVRDG VVLEPFRLQH NLAVSNHVFQ LRDSVYKTLI MRPDLELQFK CYHHEDRQMN
481TNWPASVQVS VNATPLTIER GDNKTSHKPL YLKHVCQPGR NTIQITVTAC CCSHLFVLQL
541VHRPSVRSVL QGLLKKRLLP AEHCITKIKR NFSSGTIPGT PGPNGEDGVE QTAIKVSLKC
601PITFRRIQLP ARGHDCRHIQ CFDLESYLQL NCERGTWRCP VCNKTALLEG LEVDQYMLGI
661LIYIQNSDYE EITIDPTCSW KPVPVKPDMH IKEEPDGPAL KRCRTVSPAH VLMPSVMEMI
721AALGPGAAPF APLQPPSVPA PSDYPGQGSS FLGPGTFPES FPPTTPSTPT LAEFTPGPPP
781ISYQSDIPSS LLTSEKSTAC LPSQMAPAGH LDPTHNPGTP GLHTSNLGAP PGPQLHHSNP
841PPASRQSLGQ ASLGPTGELA FSPATGVMGP PSMSGAGEAP EPALDLLPEL TNPDELLSYL
901GPPDLPTNNN DDLLSLFENN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

920 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.