Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZIMP7 / ZMIZ2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109215-100
Catalog Number: LS-G109215
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZMIZ2 (NM_031449), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NET27 | HZIMP7 | PIAS-like protein Zimp7 | TRAFIP20 | ZIMP7 | KIAA1886 | ZMIZ2
- Origin Species
- Human
- NCBI GenBank
- BC021924
- SwissProt
- Q8NF64
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 96.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNSMNPMKPA LPPAPHGDGS FAYESVPWQQ SATQPAGSLS VVTTVWGVGN ATQSQVLGNP61MGPAGSPSGS SMMPGVAGGS SALTSPQCLG QQAFAEGGAN KGYVQQGVYS RGGYPGAPGF121TTGYAGGPGG LGLPSHAARP STDFTQAAAA AAVAAAAATA TATATATVAA LQEKQSQELS181QYGAMGAGQS FNSQFLQHGG PRGPSVPAGM NPTGIGGVMG PSGLSPLAMN PTRAAGMTPL241YAGQRLPQHG YPGPPQAQPL PRQGVKRTYS EVYPGQQYLQ GGQYAPSTAQ FAPSPGQPPA301PSPSYPGHRL PLQQGMTQSL SVPGPTGLHY KPTEQFNGQG ASFNGGSVSY SQPGLSGPTR361SIPGYPSSPL PGNPTPPMTP SSSVPYMSPN QEVKSPFLPD LKPNLNSLHS SPSGSGPCDE421LRLTFPVRDG VVLEPFRLQH NLAVSNHVFQ LRDSVYKTLI MRPDLELQFK CYHHEDRQMN481TNWPASVQVS VNATPLTIER GDNKTSHKPL YLKHVCQPGR NTIQITVTAC CCSHLFVLQL541VHRPSVRSVL QGLLKKRLLP AEHCITKIKR NFSSGTIPGT PGPNGEDGVE QTAIKVSLKC601PITFRRIQLP ARGHDCRHIQ CFDLESYLQL NCERGTWRCP VCNKTALLEG LEVDQYMLGI661LIYIQNSDYE EITIDPTCSW KPVPVKPDMH IKEEPDGPAL KRCRTVSPAH VLMPSVMEMI721AALGPGAAPF APLQPPSVPA PSDYPGQGSS FLGPGTFPES FPPTTPSTPT LAEFTPGPPP781ISYQSDIPSS LLTSEKSTAC LPSQMAPAGH LDPTHNPGTP GLHTSNLGAP PGPQLHHSNP841PPASRQSLGQ ASLGPTGELA FSPATGVMGP PSMSGAGEAP EPALDLLPEL TNPDELLSYL901GPPDLPTNNN DDLLSLFENNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
920 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.