LSBio
Human TLR4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Human TLR4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G81918-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TLR4(TLR4) (NM_138554) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CD284 | CD284 antigen | Homolog of Drosophila toll | TLR-4 | TOLL | ARMD10 | TLR4 | Toll-like receptor 4 | HToll
Origin Species
Human
NCBI GenBank
NM_138554
SwissProt
O00206

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSASRLAGTL IPAMAFLSCV RPESWEPCVE VVPNITYQCM ELNFYKIPDN LPFSTKNLDL
61SFNPLRHLGS YSFFSFPELQ VLDLSRCEIQ TIEDGAYQSL SHLSTLILTG NPIQSLALGA
121FSGLSSLQKL VAVETNLASL ENFPIGHLKT LKELNVAHNL IQSFKLPEYF SNLTNLEHLD
181LSSNKIQSIY CTDLRVLHQM PLLNLSLDLS LNPMNFIQPG AFKEIRLHKL TLRNNFDSLN
241VMKTCIQGLA GLEVHRLVLG EFRNEGNLEK FDKSALEGLC NLTIEEFRLA YLDYYLDDII
301DLFNCLTNVS SFSLVSVTIE RVKDFSYNFG WQHLELVNCK FGQFPTLKLK SLKRLTFTSN
361KGGNAFSEVD LPSLEFLDLS RNGLSFKGCC SQSDFGTTSL KYLDLSFNGV ITMSSNFLGL
421EQLEHLDFQH SNLKQMSEFS VFLSLRNLIY LDISHTHTRV AFNGIFNGLS SLEVLKMAGN
481SFQENFLPDI FTELRNLTFL DLSQCQLEQL SPTAFNSLSS LQVLNMSHNN FFSLDTFPYK
541CLNSLQVLDY SLNHIMTSKK QELQHFPSSL AFLNLTQNDF ACTCEHQSFL QWIKDQRQLL
601VEVERMECAT PSDKQGMPVL SLNITCQMNK TIIGVSVLSV LVVSVVAVLV YKFYFHLMLL
661AGCIKYGRGE NIYDAFVIYS SQDEDWVRNE LVKNLEEGVP PFQLCLHYRD FIPGVAIAAN
721IIHEGFHKSR KVIVVVSQHF IQSRWCIFEY EIAQTWQFLS SRAGIIFIVL QKVEKTLLRQ
781QVELYRLLSR NTYLEWEDSV LGRHIFWRRL RKALLDGKSW NPEGTVGTGC NWQEATSI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

838 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.