Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TJP1 / ZO-1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression System	Human Embryonic Kidney 293T
Format:	Lyophilized

$220.00each

Catalog Number: LS-G74207-20

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Overview

Description: This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TJP1 (NM_003257) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category: Proteins & Peptides > Proteins > Lysates
Alternative Names: ZO-1 | Zona occludens protein 1 | Zona occludens 1 | Zonula occludens protein 1 | Tight junction protein 1 | ZO1 | Tight junction protein ZO-1 | TJP1 | Zonula occludens 1 protein
Origin Species: Human
NCBI GenBank: NM_003257
SwissProt: Q07157

Protein

Expression System: Human Embryonic Kidney 293T
Fusion Tag: c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight: 195.3 kDa
Protein Type: Lysate
Amino Acid Sequence

Format

Purity: Unpurified
Conjugate/Label: Unconjugated
Endotoxin Level: Not Tested
Format: Lyophilized
Formulation: Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4