Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Anti-ZO-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC-plus™ Polyclonal Rabbit anti-Human TJP1 / ZO-1 Antibody (aa463-1109, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Dog, Guinea Pig, Human, Mouse, Rat |
| Format: | Liquid |
$495.00each
Catalog Number: LS-B2129-50
Catalog Number: LS-B2129
For bulk orders or custom formulations:
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- Description
- ZO-1 antibody LS-B2129 is an unconjugated rabbit polyclonal antibody to ZO-1 (TJP1) (aa463-1109) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
- Application
- ELISA, IHC, IHC-F, IHC-P, IP, WB
- Reactivity
- Can, GP, Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ZO-1 | Zona occludens protein 1 | Zona occludens 1 | Zonula occludens protein 1 | Tight junction protein 1 | ZO1 | Tight junction protein ZO-1 | TJP1 | Zonula occludens 1 protein
- Recommended Dilution: IHC-P
- 5 µg/ml
- Recommended Dilution: WB
- 0.5 - 2 µg/ml
- UniProt Number
- Q07157
- NCBI GenBank
- NM_003257 | NP_003248.3
- SwissProt
- Q07157
Target
- Target
- TJP1 / ZO-1
- Antigen Species
- Human
- Specificity
- Recognizes human ZO-1alpha+ and ZO- alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western blots, but potential reactivity with these proteins has not been fully characterized. Predicted species cross-reactivity: mouse, rat, guinea pig, canine and chicken (moderate reactivity). Please note that cross-reactivity with predicted species is only predicted and is not guaranteed.
- Epitope
- Recognizes human ZO-1alpha+ and ZO- alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western blots, but potential reactivity with these proteins has not been fully characterized. Predicted species cross-reactivity: mouse, rat, guinea pig, canine and chicken (moderate reactivity). Please note that cross-reactivity with predicted species is only predicted and is not guaranteed.
- Gene Name
- TJP1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- A 69 kDa fusion protein corresponding to aa 463-1109 of human ZO-1 cDNA. This sequence lies N-Terminus to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide
- Concentration
- 0.25 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Low doses of celecoxib attenuate gut barrier failure during experimental peritonitis. Short SS, Wang J, Castle SL, Fernandez GE, Smiley N, Zobel M, Pontarelli EM, Papillon SC, Grishin AV, Ford HR. Laboratory investigation; a journal of technical methods and pathology. 2013;93:1265-75. PubMed: 24126890
